ArticleApplied and environmental microbiology2011
Global genome transcription profiling of Bifidobacterium bifidum PRL2010 under in vitro conditions and identification of reference genes for quantitative real-time PCR.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mucin O-glycan degradation by GH101 underpins mucosal persistence of Bifidobacterium bifidum PRL2010.Applied microbiology and biotechnology · 2026Article
- Aromatic lactic acid production byApplied and environmental microbiology · 2026Article
- Understanding the interaction between melatonin and bifidobacteria.Microbiome research reports · 2025Article
- The role of teichoic acids of bifidobacteria in driving the interaction with the human host.Frontiers in microbiology · 2025Article
- Molecular cross-talk among human intestinal bifidobacteria as explored by a human gut model.Frontiers in microbiology · 2024Article
- The Gene Expression Profile Differs in Growth Phases of theMicroorganisms · 2022Article
- Transcriptome analysis revealed growth phase-associated changes of a centenarian-originated probiotic Bifidobacterium animalis subsp. lactis A6.BMC microbiology · 2022Article
- The infant gut microbiome as a microbial organ influencing host well-being.Italian journal of pediatrics · 2020Review
- Bifidobacterial Transfer from Mother to Child as Examined by an Animal Model.Microorganisms · 2019Article
- Article
- The Sortase-Dependent Fimbriome of the Genus Bifidobacterium: Extracellular Structures with Potential To Modulate Microbe-Host Dialogue.Applied and environmental microbiology · 2017Article
- Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor.Scientific reports · 2015Article
- Mutual Cross-Feeding Interactions between Bifidobacterium longum subsp. longum NCC2705 and Eubacterium rectale ATCC 33656 Explain the Bifidogenic and Butyrogenic Effects of Arabinoxylan Oligosaccharides.Applied and environmental microbiology · 2015Article
- Exploring Amino Acid Auxotrophy in Bifidobacterium bifidum PRL2010.Frontiers in microbiology · 2015Article
- Glycan cross-feeding activities between bifidobacteria under in vitro conditions.Frontiers in microbiology · 2015Article
- Genomic characterization and transcriptional studies of the starch-utilizing strain Bifidobacterium adolescentis 22L.Applied and environmental microbiology · 2014Article
- Bifidobacterium bifidum PRL2010 modulates the host innate immune response.Applied and environmental microbiology · 2014Article
- A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2012Article
- Analysis of predicted carbohydrate transport systems encoded by Bifidobacterium bifidum PRL2010.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bifidobacteria have attracted significant scientific attention due to their perceived role as health-promoting microorganisms, although the genetics of the bacterial group is still underexplored. In this study, we investigated the transcriptome of Bifidobacterium bifidum PRL2010 during in vitro growth by microarray technology. When B. bifidum PRL2010 was grown in liquid broth, 425 of the 1,644 PRL2010 genes represented on the array were expressed in at least one of the three investigated growth phases, i.e., the lag, exponential, and stationary phases. These transcriptional analyses identified a core in vitro transcriptome encompassing 150 genes that are expressed in all phases. A proportion of these genes were further investigated as potential reference genes by quantitative real-time reverse transcription-PCR (qRT-PCR) assays. Their expression stability was evaluated under different growth conditions, which included cultivation on different carbon sources, exposure to environmental stresses (thermal, acidic, and osmotic), and growth phases. Our analyses validated six reference genes suitable for normalizing mRNA expression levels in qRT-PCR experiments applied to bifidobacteria.
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