ArticleApplied and environmental microbiology2011
A novel hydrolase identified by genomic-proteomic analysis of phenylurea herbicide mineralization by Variovorax sp. strain SRS16.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 76 citations in OpenAlex.
- Enzymatic approaches to nicotinic acid synthesis: recent advances and future prospects.Frontiers in bioengineering and biotechnology · 2025Review
- Omic-driven strategies to unveil microbiome potential for biodegradation of plastics: a review.Archives of microbiology · 2024Review
- Characterization of the 3,4-Dichloroaniline Degradation Gene Cluster inMicroorganisms · 2024Article
- Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes.Microbiology spectrum · 2022Article
- Emerging Strategies for the Bioremediation of the Phenylurea Herbicide Diuron.Frontiers in microbiology · 2021Review
- Strategies for EnhancingFrontiers in bioengineering and biotechnology · 2021Article
- Comparative Genomics Suggests Mechanisms of Genetic Adaptation toward the Catabolism of the Phenylurea Herbicide Linuron in Variovorax.Genome biology and evolution · 2020Article
- Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep.Microbial cell factories · 2020Article
- PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera.Frontiers in microbiology · 2020Article
- Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors.Scientific reports · 2018Article
- Metabolic and Evolutionary Insights in the Transformation of Diphenylamine by aFrontiers in microbiology · 2018Article
- Metabolic pathway and cell adaptation mechanisms revealed through genomic, proteomic and transcription analysis of a Sphingomonas haloaromaticamans strain degrading ortho-phenylphenol.Scientific reports · 2017Article
- Novel insights into the metabolic pathway of iprodione by soil bacteria.Environmental science and pollution research international · 2017Article
- Functional Redundancy of Linuron Degradation in Microbial Communities in Agricultural Soil and Biopurification Systems.Applied and environmental microbiology · 2016Article
- Diuron tolerance and potential degradation by pelagic microbiomes in the Great Barrier Reef lagoon.PeerJ · 2016Article
- Effect of the soil type on the microbiome in the rhizosphere of field-grown lettuce.Frontiers in microbiology · 2014Article
- The novel bacterial N-demethylase PdmAB is responsible for the initial step of N,N-dimethyl-substituted phenylurea herbicide degradation.Applied and environmental microbiology · 2013Article
- HylA, an alternative hydrolase for initiation of catabolism of the phenylurea herbicide linuron in Variovorax sp. strains.Applied and environmental microbiology · 2013Article
- A metagenomic study highlights phylogenetic proximity of quorum-quenching and xenobiotic-degrading amidases of the AS-family.PloS one · 2013Article
- Cloning of a novel arylamidase gene from Paracoccus sp. strain FLN-7 that hydrolyzes amide pesticides.Applied and environmental microbiology · 2012Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
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Abstract
The soil bacterial isolate Variovorax sp. strain SRS16 mineralizes the phenylurea herbicide linuron. The proposed pathway initiates with hydrolysis of linuron to 3,4-dichloroaniline (DCA) and N,O-dimethylhydroxylamine, followed by conversion of DCA to Krebs cycle intermediates. Differential proteomic analysis showed a linuron-dependent upregulation of several enzymes that fit into this pathway, including an amidase (LibA), a multicomponent chloroaniline dioxygenase, and enzymes associated with a modified chlorocatechol ortho-cleavage pathway. Purified LibA is a monomeric linuron hydrolase of ∼55 kDa with a K(m) and a V(max) for linuron of 5.8 μM and 0.16 nmol min⁻¹, respectively. This novel member of the amidase signature family is unrelated to phenylurea-hydrolyzing enzymes from Gram-positive bacteria and lacks activity toward other tested phenylurea herbicides. Orthologues of libA are present in all other tested linuron-degrading Variovorax strains with the exception of Variovorax strains WDL1 and PBS-H4, suggesting divergent evolution of the linuron catabolic pathway in different Variovorax strains. The organization of the linuron degradation genes identified in the draft SRS16 genome sequence indicates that gene patchwork assembly is at the origin of the pathway. Transcription analysis suggests that a catabolic intermediate, rather than linuron itself, acts as effector in activation of the pathway. Our study provides the first report on the genetic organization of a bacterial pathway for complete mineralization of a phenylurea herbicide and the first report on a linuron hydrolase in Gram-negative bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.