ArticleProtoplasma2012
Genetic and biochemical characterization of somatic hybrids between Bupleurum scorzonerifolium and Gentianopsis paludosa.
Article in Protoplasma, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 9 citations in OpenAlex.
- Biotechnological interventions and genetic diversity assessment in Swertia sp.: a myriad source of valuable secondary metabolites.Applied microbiology and biotechnology · 2021Review
- The complete chloroplast genome of Tibetan medicineMitochondrial DNA. Part B, Resources · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultraviolet-irradiated protoplasts of Gentianopsis paludosa were fused with those of Bupleurum scorzonerifolium and 28 independent hybrid calli were identified, five of which later differentiated into plants. A genetic analysis of these calli and regenerated plants based on chromosome number, esterase, random amplified polymorphic DNA, and 5S rDNA spacer profiling showed that the majority of their nuclear genomes were represented by the recipient biparent B. scorzonerifolium. A restriction fragment length polymorphism analysis of the plastidial genomes confirmed that DNA from both biparents was present in some of the hybrids. The secondary metabolite composition of the hybrids was analyzed by a combination of high-performance liquid chromatography and gas chromatography-mass spectrometry analysis. The content of oleanolic acid in two of the hybrid lines was substantially higher than in the donor G. paludosa, while that of swertiamarin was equal to that in G. paludosa in two of the six hybrids analyzed. A number of both G. paludosa and B. scorzonerifolium specific compounds were detected in the three hybrids analyzed by GC-MS as were several not present in either of the biparents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.