ArticleAsian journal of andrology2012
Identification of testosterone-/androgen receptor-regulated genes in mouse Sertoli cells.
Article in Asian journal of andrology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Toxicant exposure during pregnancy increases protective proteins in the dam and a sexually dimorphic response in the fetus.Toxicology and applied pharmacology · 2021Article
- PMCA4 gene expression is regulated by the androgen receptor in the mouse testis during spermatogenesis.Molecular medicine reports · 2021Article
- Single-Cell RNA Sequencing Defines the Regulation of Spermatogenesis by Sertoli-Cell Androgen Signaling.Frontiers in cell and developmental biology · 2021Article
- Regulation of Human Spermatogenesis.Advances in experimental medicine and biology · 2021Article
- Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression.Iranian journal of basic medical sciences · 2017Article
- Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor.International journal of molecular sciences · 2015Article
- Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Long-term culture and analysis of cashmere goat Sertoli cells.In vitro cellular & developmental biology. Animal · 2014Article
- Genome-wide identification of AR-regulated genes translated in Sertoli cells in vivo using the RiboTag approach.Molecular endocrinology (Baltimore, Md.) · 2014Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Androgen and androgen receptor (AR) play important roles in male spermatogenesis and fertility, yet detailed androgen/AR signals in Sertoli cells remain unclear. To identify AR target genes in Sertoli cells, we analyzed the gene expression profiles of testis between mice lacking AR in Sertoli cells (S-AR(-/y)) and their littermate wild-type (WT) mice. Digital gene expression analysis identified 2276 genes downregulated and 2865 genes upregulated in the S-AR(-/y) mice testis compared to WT ones. To further nail down the difference within Sertoli cells, we first constructed Sertoli cell line TM4 with stably transfected AR (named as TM4/AR) and found androgens failed to transactivate AR in Sertoli TM4 and TM4/AR cells. Interestingly, additional transient transfection of AR-cDNA resulted in significant androgen responsiveness with TM4/AR cells showing 10 times more androgen sensitivity than TM4 cells. In the condition where maximal androgen response was demonstrated, we then analyzed gene expression and found the expression levels of 2313 genes were changed more than twofold by transient transfection of AR-cDNA in the presence of testosterone. Among these genes, 603 androgen-/AR-regulated genes, including 164 upregulated and 439 downregulated, were found in both S-AR(-/y) mice testis and TM4/AR cells. Using informatics analysis, the gene ontology was applied to analyze these androgen-/AR-regulated genes to predict the potential roles of androgen/AR in the process of spermatogenesis. Together, using gene analysis in both S-AR(-/y) mice testis and TM4/AR cells may help us to better understand the androgen/AR signals in Sertoli cells and their influences in spermatogenesis.
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