ArticleJournal of bacteriology2011
Malate-mediated carbon catabolite repression in Bacillus subtilis involves the HPrK/CcpA pathway.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Secondary Metabolite Biosynthesis and Regulatory Role of Sucrose inMicroorganisms · 2026Review
- Sucrose and malic acid in the tobacco plant induceJournal of bacteriology · 2025Article
- Efficacious Extraction and Purification Technique of a Potential Antimycobacterial Bacteriocin Produced byIndian journal of microbiology · 2023Article
- Differences in Microbial Communities Stimulated by Malic Acid Have the Potential to Improve Nutrient Absorption and Fruit Quality of Grapes.Frontiers in microbiology · 2022Article
- Surfactin, a quorum sensing signal molecule, globally affects the carbon metabolism inMetabolic engineering communications · 2021Article
- Kinetic Modeling and Meta-Analysis of theMicroorganisms · 2021Article
- Malate-Dependent Carbon Utilization Enhances Central Metabolism and Contributes to Biological Fitness ofFrontiers in microbiology · 2019Article
- A metabolic checkpoint protein GlmR is important for diverting carbon into peptidoglycan biosynthesis in Bacillus subtilis.PLoS genetics · 2018Article
- Capacity for instantaneous catabolism of preferred and non-preferred carbon sources in Escherichia coli and Bacillus subtilis.Scientific reports · 2018Article
- Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh inThe Journal of biological chemistry · 2018Article
- Optimal resource allocation enables mathematical exploration of microbial metabolic configurations.Journal of mathematical biology · 2017Article
- Molecular and Physiological Logics of the Pyruvate-Induced Response of a Novel Transporter inmBio · 2017Article
- Contrasting mechanisms of growth in two model rod-shaped bacteria.Nature communications · 2017Article
- Regulation of the rhaEWRBMA Operon Involved in l-Rhamnose Catabolism through Two Transcriptional Factors, RhaR and CcpA, in Bacillus subtilis.Journal of bacteriology · 2015Article
- The phosphoenolpyruvate:sugar phosphotransferase system is involved in sensitivity to the glucosylated bacteriocin sublancin.Antimicrobial agents and chemotherapy · 2015Article
- Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs--commonalities and differences.RNA (New York, N.Y.) · 2014Article
- The sensor kinase DctS forms a tripartite sensor unit with DctB and DctA for sensing C4-dicarboxylates in Bacillus subtilis.Journal of bacteriology · 2014Article
- Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.RNA biology · 2014Review
- ABC transport is inactivated by the PTS(Ntr) under potassium limitation in Rhizobium leguminosarum 3841.PloS one · 2013Article
- Fine-tuned transcriptional regulation of malate operons in Enterococcus faecalis.Applied and environmental microbiology · 2012Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most organisms can choose their preferred carbon source from a mixture of nutrients. This process is called carbon catabolite repression. The Gram-positive bacterium Bacillus subtilis uses glucose as the preferred source of carbon and energy. Glucose-mediated catabolite repression is caused by binding of the CcpA transcription factor to the promoter regions of catabolic operons. CcpA binds DNA upon interaction with its cofactors HPr(Ser-P) and Crh(Ser-P). The formation of the cofactors is catalyzed by the metabolite-activated HPr kinase/phosphorylase. Recently, it has been shown that malate is a second preferred carbon source for B. subtilis that also causes catabolite repression. In this work, we addressed the mechanism by which malate causes catabolite repression. Genetic analyses revealed that malate-dependent catabolite repression requires CcpA and its cofactors. Moreover, we demonstrate that HPr(Ser-P) is present in malate-grown cells and that CcpA and HPr interact in vivo in the presence of glucose or malate but not in the absence of a repressing carbon source. The formation of the cofactor HPr(Ser-P) could be attributed to the concentrations of ATP and fructose 1,6-bisphosphate in cells growing with malate. Both metabolites are available at concentrations that are sufficient to stimulate HPr kinase activity. The adaptation of cells to environmental changes requires dynamic metabolic and regulatory adjustments. The repression strength of target promoters was similar to that observed in steady-state growth conditions, although it took somewhat longer to reach the second steady-state of expression when cells were shifted to malate.
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