ArticleMetabolic engineering2011
Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products.
Article in Metabolic engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Microbial synthesis of bacitracin: Recent progress, challenges, and prospects.Synthetic and systems biotechnology · 2023Review
- Interaction of Periplasmic Fab Production and Intracellular Redox Balance inACS synthetic biology · 2022Article
- Improved production of 2,3-butanediol and isobutanol by engineering electron transport chain in Escherichia coli.Microbial biotechnology · 2021Article
- Metabolic characterisation ofRSC advances · 2020Article
- Aerobic radical polymerization mediated by microbial metabolism.Nature chemistry · 2020Article
- Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case ofFrontiers in bioengineering and biotechnology · 2019Review
- A genome-wide screen inThe Journal of biological chemistry · 2017Article
- Engineering redox homeostasis to develop efficient alcohol-producing microbial cell factories.Microbial cell factories · 2017Review
- Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes.Journal of industrial microbiology & biotechnology · 2017Review
- Engineering microaerobic metabolism of E. coli for 1,2-propanediol production.Journal of industrial microbiology & biotechnology · 2015Article
- Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli.Metabolic engineering · 2015Article
- Metabolic engineering of carbon and redox flow in the production of small organic acids.Journal of industrial microbiology & biotechnology · 2015Review
- Genome engineering for improved recombinant protein expression in Escherichia coli.Microbial cell factories · 2014Review
- ubiJ, a new gene required for aerobic growth and proliferation in macrophage, is involved in coenzyme Q biosynthesis in Escherichia coli and Salmonella enterica serovar Typhimurium.Journal of bacteriology · 2014Article
- Ubiquinone and menaquinone electron carriers represent the yin and yang in the redox regulation of the ArcB sensor kinase.Journal of bacteriology · 2013Article
- Novel dehalogenase mechanism for 2,3-dichloro-1-propanol utilization in Pseudomonas putida strain MC4.Applied and environmental microbiology · 2012Article
- Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals.Computational and structural biotechnology journal · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Optimizing the productivity of bioengineered strains requires balancing ATP generation and carbon atom conservation through fine-tuning cell respiration and metabolism. Traditional approaches manipulate cell respiration by altering air feeding, which are technically difficult especially in large bioreactors. An approach based on genetic regulation may better serve this purpose. With excess oxygen supply to the culture, we efficiently manipulated Escherichia coli cell respiration by adding different amount of coenzyme Q1 to strains lacking the ubiCA genes, which encode two critical enzymes for ubiquinone synthesis. As a proof-of-concept, the metabolic effect of the ubiCA gene knockout and coenzyme Q1 supplementation were characterized, and the metabolic profiles of the experimental strains showed clear correlations with coenzyme Q1 concentrations. Further proof-of-principle experiments were performed to illustrate that the approach can be used to optimize cell respiration for the production of chemicals of interest such as ethanol. This study showed that controlled respiration through genetic manipulation can be exploited to allow much larger operating windows for reduced product formation even under fully aerobic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.