ArticleCurrent biology : CB2011
Blimp1 expression predicts embryonic stem cell development in vitro.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 1 citations in OpenAlex.
- Inducing Pluripotency in Somatic Cells: Historical Perspective and Recent Advances.International journal of stem cells · 2024Review
- Genome-wide profiling of angiogenic cis-regulatory elements unravels cis-regulatory SNPs for vascular abnormality.Scientific data · 2024Article
- PRDM12 in Health and Diseases.International journal of molecular sciences · 2021Review
- Review
- Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription.Genes · 2019Review
- Article
- Transition of inner cell mass to embryonic stem cells: mechanisms, facts, and hypotheses.Cellular and molecular life sciences : CMLS · 2019Review
- Recent advances in understanding intestinal stem cell regulation.F1000Research · 2019Review
- Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm.Genome biology · 2016Article
- Evolution of Prdm Genes in Animals: Insights from Comparative Genomics.Molecular biology and evolution · 2016Article
- Pluripotent Stem Cells: Current Understanding and Future Directions.Stem cells international · 2016Review
- Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro.International journal of molecular medicine · 2015Article
- The birth of embryonic pluripotency.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification.Nature cell biology · 2014Article
- Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells.Stem cell reports · 2014Article
- Derivation of human embryonic stem cells using a post-inner cell mass intermediate.Nature protocols · 2013Article
- Lessons from human teratomas to guide development of safe stem cell therapies.Nature biotechnology · 2012Review
- Pluripotency in the embryo and in culture.Cold Spring Harbor perspectives in biology · 2012Review
- The germ cell determinant Blimp1 is not required for derivation of pluripotent stem cells.Cell stem cell · 2012Article
- The reciprocal relationship between primordial germ cells and pluripotent stem cells.Journal of molecular medicine (Berlin, Germany) · 2012Review
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4 authors at 3 institutions in 2 countries.
Funding
Abstract
Despite recent critical insights into the pluripotent state of embryonic stem cells (ESCs), there is little agreement over the inaugural and subsequent steps leading to its generation [1-4]. Here we show that inner cell mass (ICM)-generated cells expressing Blimp1, a key transcriptional repressor of the somatic program during germ cell specification [5, 6], emerge on day 2 of blastocyst culture. Single-cell gene expression profiling indicated that many of these Blimp1-positive cells coexpress other genes typically associated with early germ cell specification. When genetically traced in vitro, these cells acquired properties normally associated with primordial germ cells. Importantly, fate-mapping experiments revealed that ESCs commonly arise from Blimp1-positive precursors; indeed, prospective sorting of such cells from ICM outgrowths increased the rate of ESC derivation more than 9-fold. Finally, using genetic ablation or distinct small molecules [7, 8], we show that epiblast cells can become ESCs without first acquiring Blimp1 positivity. Our findings suggest that the germ cell-like state is facultative for the stabilization of pluripotency in vitro. Thus, the association of Blimp1 expression with ESC development furthers understanding of how the pluripotent state of these cells is established in vitro and suggests a means to enhance the generation of new stem cell lines from blastocysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.