ArticlePloS one2011
A system for performing high throughput assays of synaptic function.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- CRISPR-Enabled functional genomics in hPSCs-derived neural models for autism spectrum disorder.Metabolic brain disease · 2026Review
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Article
- High-throughput Analysis of Synaptic Activity in Electrically Stimulated Neuronal Cultures.Neuroinformatics · 2021Article
- Screening for modulators of neural network activity in 3D human iPSC-derived cortical spheroids.PloS one · 2020Article
- Cell death assays for neurodegenerative disease drug discovery.Expert opinion on drug discovery · 2019Review
- Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture.Frontiers in neuroscience · 2018Review
- A high-throughput model for investigating neuronal function and synaptic transmission in cultured neuronal networks.Scientific reports · 2017Article
- Bringing new dimensions to drug discovery screening: impact of cellular stimulation technologies.Drug discovery today · 2017Review
- Optogenetic Approaches to Drug Discovery in Neuroscience and Beyond.Trends in biotechnology · 2017Review
- Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.eLife · 2016Article
- Concise Review: Progress and Challenges in Using Human Stem Cells for Biological and Therapeutics Discovery: Neuropsychiatric Disorders.Stem cells (Dayton, Ohio) · 2016Review
- Micro-electrode array recordings reveal reductions in both excitation and inhibition in cultured cortical neuron networks lacking Shank3.Molecular psychiatry · 2016Article
- In vitro clinical trials: the future of cell-based profiling.Frontiers in pharmacology · 2014Article
- High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.PloS one · 2014Article
- Working memory impairment in calcineurin knock-out mice is associated with alterations in synaptic vesicle cycling and disruption of high-frequency synaptic and network activity in prefrontal cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Experimental models for identifying modifiers of polyglutamine-induced aggregation and neurodegeneration.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2013Article
- Meeting report: using stem cells for biological and therapeutics discovery in mental illness, April 2012.Stem cells translational medicine · 2013Article
- A neuron-based screening platform for optimizing genetically-encoded calcium indicators.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Unbiased, high-throughput screening has proven invaluable for dissecting complex biological processes. Application of this general approach to synaptic function would have a major impact on neuroscience research and drug discovery. However, existing techniques for studying synaptic physiology are labor intensive and low-throughput. Here, we describe a new high-throughput technology for performing assays of synaptic function in primary neurons cultured in microtiter plates. We show that this system can perform 96 synaptic vesicle cycling assays in parallel with high sensitivity, precision, uniformity, and reproducibility and can detect modulators of presynaptic function. By screening libraries of pharmacologically defined compounds on rat forebrain cultures, we have used this system to identify novel effects of compounds on specific aspects of presynaptic function. As a system for unbiased compound as well as genomic screening, this technology has significant applications for basic neuroscience research and for the discovery of novel, mechanism-based treatments for central nervous system disorders.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.