ArticlePloS one2011
Gene expression of human lung cancer cell line CL1-5 in response to a direct current electric field.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor.Biomicrofluidics · 2024Article
- Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression.Biochemistry and biophysics reports · 2024Article
- Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells.Advanced healthcare materials · 2022Review
- Understanding the unimodal distributions of cancer occurrence rates: it takes two factors for a cancer to occur.Briefings in bioinformatics · 2021Article
- Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.Bioelectricity · 2021Article
- Hub Proteins Involved in RAW 264.7 Macrophages Exposed to Direct Current Electric Field.International journal of molecular sciences · 2020Article
- Cellular processes involved in lung cancer cells exposed to direct current electric field.Scientific reports · 2020Article
- Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells.Scientific reports · 2019Article
- Electrotaxis of Glioblastoma and Medulloblastoma Spheroidal Aggregates.Scientific reports · 2019Article
- A microfluidic chip-based co-culture of fibroblast-like synoviocytes with osteoblasts and osteoclasts to test bone erosion and drug evaluation.Royal Society open science · 2018Article
- Studying Electrotaxis in Microfluidic Devices.Sensors (Basel, Switzerland) · 2017Review
- Article
- Uniform electric field generation in circular multi-well culture plates using polymeric inserts.Scientific reports · 2016Article
- Article
- Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip.Journal of visualized experiments : JoVE · 2015Article
- Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.Biosensors · 2015Article
- Transmembrane voltage potential of somatic cells controls oncogene-mediated tumorigenesis at long-range.Oncotarget · 2014Article
- Engineered culture models for studies of tumor-microenvironment interactions.Annual review of biomedical engineering · 2013Review
- Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under direct-current electric field stimulation.PloS one · 2013Article
- Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds.Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundElectrotaxis is the movement of adherent living cells in response to a direct current (dc) electric field (EF) of physiological strength. Highly metastatic human lung cancer cells, CL1-5, exhibit directional migration and orientation under dcEFs. To understand the transcriptional response of CL1-5 cells to a dcEF, microarray analysis was performed in this study. METHODOLOGY/PRINCIPAL
findingsA large electric-field chip (LEFC) was designed, fabricated, and used in this study. CL1-5 cells were treated with the EF strength of 0 mV/mm (the control group) and 300 mV/mm (the EF-treated group) for two hours. Signaling pathways involving the genes that expressed differently between the two groups were revealed. It was shown that the EF-regulated genes highly correlated to adherens junction, telomerase RNA component gene regulation, and tight junction. Some up-regulated genes such as ACVR1B and CTTN, and some down-regulated genes such as PTEN, are known to be positively and negatively correlated to cell migration, respectively. The protein-protein interactions of adherens junction-associated EF-regulated genes suggested that platelet-derived growth factor (PDGF) receptors and ephrin receptors may participate in sensing extracellular electrical stimuli. We further observed a high percentage of significantly regulated genes which encode cell membrane proteins, suggesting that dcEF may directly influence the activity of cell membrane proteins in signal transduction. CONCLUSIONS/SIGNIFICANCE: In this study, some of the EF-regulated genes have been reported to be essential whereas others are novel for electrotaxis. Our result confirms that the regulation of gene expression is involved in the mechanism of electrotactic response.
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