ArticlePloS one2011
Elution of labile fluorescent dye from nanoparticles during biological use.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Translocation of (ultra)fine particles and nanoparticles across the placenta; a systematic review on the evidence of in vitro, ex vivo, and in vivo studies.Particle and fibre toxicology · 2020Pooled it
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- Oral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes.Environmental health perspectives · 2023Article
- Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lungFrontiers in toxicology · 2023Review
- DNA-Immobilized Fluorescent Polystyrene Nanoparticles as Probes with Tunable Detection Limits.ACS omega · 2022Article
- The use of advanced spectral imaging to reveal nanoparticle identity in biological samples.Nanoscale · 2022Article
- Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties.Pharmaceutics · 2021Article
- Harnessing PET to track micro- and nanoplastics in vivo.Scientific reports · 2021Article
- Label-free spectral imaging to study drug distribution and metabolism in single living cells.Scientific reports · 2021Article
- Micro and Nanoplastics Identification: Classic Methods and Innovative Detection Techniques.Frontiers in toxicology · 2021Review
- Methods, models, mechanisms and metadata: Introducing the Nanotoxicology collection at F1000Research.F1000Research · 2021Article
- Monitoring the Fate of Orally Administered PLGA Nanoformulation for Local Delivery of Therapeutic Drugs.Pharmaceutics · 2019Article
- Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers.Beilstein journal of nanotechnology · 2017Article
- Transient loading of CD34International journal of nanomedicine · 2017Article
- Understanding Critical Quality Attributes for Nanocrystals from Preparation to Delivery.Molecules (Basel, Switzerland) · 2015Review
- Bidirectional Transfer Study of Polystyrene Nanoparticles across the Placental Barrier in an ex Vivo Human Placental Perfusion Model.Environmental health perspectives · 2015Article
- Transfer studies of polystyrene nanoparticles in theScience and technology of advanced materials · 2015Article
- 'Bio-nano interactions: new tools, insights and impacts': summary of the Royal Society discussion meeting.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Protein Corona Prevents TiO2 Phototoxicity.PloS one · 2015Article
- Tracking translocation of industrially relevant engineered nanomaterials (ENMs) across alveolar epithelial monolayers in vitro.Nanotoxicology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells act as extremely efficient filters for elution of unbound fluorescent tags or impurities associated with nanoparticles, including those that cannot be removed by extensive cleaning. This has consequences for quantification of nanoparticle uptake and sub-cellular localization in vitro and in vivo as a result of the presence of significant amount of labile dye even following extensive cleaning by dialysis. Polyacrylamide gel electrophoresis (PAGE) can be used to monitor the elution of unbound fluorescent probes from nanoparticles, either commercially available or synthesized in-house, and to ensure their complete purification for biological studies, including cellular uptake and sub-cellular localisation. Very different fluorescence distribution within cells is observed after short dialysis times versus following extensive dialysis against a solvent in which the free dye is more soluble, due to the contribution from free dye. In the absence of an understanding of the presence of residual free dye in (most) labeled nanoparticle solutions, the total fluorescence intensity in cells following exposure to nanoparticle solutions could be mis-ascribed to the presence of nanoparticles through the cell, rather than correctly assigned to either a combination of free-dye and nanoparticle-bound dye, or even entirely to free dye depending on the exposure conditions (i.e. aggregation of the particles etc). Where all of the dye is nanoparticle-bound, the particles are highly localized in sub-cellular organelles, likely lysosomes, whereas in a system containing significant amounts of free dye, the fluorescence is distributed through the cell due to the free diffusion of the molecule dye across all cellular barriers and into the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.