ArticlePLoS genetics2011
A barcode screen for epigenetic regulators reveals a role for the NuB4/HAT-B histone acetyltransferase complex in histone turnover.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- A genetic interaction between DED1 and HAT1 in Saccharomyces cerevisiae reveals a role for Hat1p in cytoplasmic RNA granule accumulation.G3 (Bethesda, Md.) · 2026Article
- Histone retention preserves epigenetic marks during heat stress-induced transcriptional memory in plants.The EMBO journal · 2023Article
- The histone code of the fungal genusMicrobial genomics · 2022Article
- Rtt109 promotes nucleosome replacement ahead of the replication fork.Genome research · 2022Article
- Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies.Scientific reports · 2021Article
- Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.Epigenetics · 2020Article
- Inhibition of transcription leads to rewiring of locus-specific chromatin proteomes.Genome research · 2020Article
- Epi-ID: Systematic and Direct Screening for Chromatin Regulators in Yeast by Barcode-ChIP-Seq.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Functional Analysis of Hif1 Histone Chaperone inG3 (Bethesda, Md.) · 2018Article
- Article
- The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.EMBO reports · 2015Article
- Regulation of histone gene transcription in yeast.Cellular and molecular life sciences : CMLS · 2014Review
- Article
- Recombination-induced tag exchange (RITE) cassette series to monitor protein dynamics in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2013Article
- Sites of acetylation on newly synthesized histone H4 are required for chromatin assembly and DNA damage response signaling.Molecular and cellular biology · 2013Article
- Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover.The Journal of biological chemistry · 2013Article
- Histone acetyl transferase 1 is essential for mammalian development, genome stability, and the processing of newly synthesized histones H3 and H4.PLoS genetics · 2013Article
- Spatiotemporal analysis of organelle and macromolecular complex inheritance.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Systematic dissection of roles for chromatin regulators in a yeast stress response.PLoS biology · 2012Article
- A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic modification of histone proteins plays a key role in regulating gene expression. However, histones themselves can also be dynamic, which potentially affects the stability of histone modifications. To determine the molecular mechanisms of histone turnover, we developed a parallel screening method for epigenetic regulators by analyzing chromatin states on DNA barcodes. Histone turnover was quantified by employing a genetic pulse-chase technique called RITE, which was combined with chromatin immunoprecipitation and high-throughput sequencing. In this screen, the NuB4/HAT-B complex, containing the conserved type B histone acetyltransferase Hat1, was found to promote histone turnover. Unexpectedly, the three members of this complex could be functionally separated from each other as well as from the known interacting factor and histone chaperone Asf1. Thus, systematic and direct interrogation of chromatin structure on DNA barcodes can lead to the discovery of genes and pathways involved in chromatin modification and dynamics.
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