ArticlePLoS pathogens2011
Blood feeding and insulin-like peptide 3 stimulate proliferation of hemocytes in the mosquito Aedes aegypti.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 112 citations in OpenAlex.
- Multiple evolutionary routes to immune diversification across animals.International immunology · 2026Review
- Recent insights in the development and functions of insect hemocytes.Current opinion in insect science · 2026Review
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Characterization of Anopheles gambiae immune cells through genetic and functional immunophenotyping.Nature communications · 2025Article
- The effects of mating and blood feeding on the immune defense of female Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2025Article
- TNF signaling mediates cellular immune function and promotes malaria parasite killing in the mosquito Anopheles gambiae.PLoS pathogens · 2025Article
- Influence of blood feeding and infection on arthropod hemocytes.Current opinion in insect science · 2025Review
- Review
- Anopheles gambiae phagocytic hemocytes promote Plasmodium falciparum infection by regulating midgut epithelial integrity.Nature communications · 2025Article
- Warmer temperature accelerates reproductive senescence in mosquitoes.Frontiers in physiology · 2025Article
- Exploring new dimensions of immune cell biology inbioRxiv : the preprint server for biology · 2024Article
- Tick hemocytes have a pleiotropic role in microbial infection and arthropod fitness.Nature communications · 2024Article
- Higher temperature accelerates the aging-dependent weakening of the melanization immune response in mosquitoes.PLoS pathogens · 2024Article
- Increased blood meal size and feeding frequency compromise Aedes aegypti midgut integrity and enhance dengue virus dissemination.PLoS neglected tropical diseases · 2023Article
- Sugar restriction and blood ingestion shape divergent immune defense trajectories in the mosquito Aedes aegypti.Scientific reports · 2023Article
- Molecular and expression characterization of insulin-like signaling in development and metabolism of Aedes albopictus.Parasites & vectors · 2023Article
- The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides.International journal of molecular sciences · 2022Review
- Insulin-like peptide 3 stimulates hemocytes to proliferate in anautogenous and facultatively autogenous mosquitoes.The Journal of experimental biology · 2022Article
- Article
- Impacts of Infectious Dose, Feeding Behavior, and Age ofPathogens (Basel, Switzerland) · 2021Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
All vector mosquito species must feed on the blood of a vertebrate host to produce eggs. Multiple cycles of blood feeding also promote frequent contacts with hosts, which enhance the risk of exposure to infectious agents and disease transmission. Blood feeding triggers the release of insulin-like peptides (ILPs) from the brain of the mosquito Aedes aegypti, which regulate blood meal digestion and egg formation. In turn, hemocytes serve as the most important constitutive defense in mosquitoes against pathogens that enter the hemocoel. Prior studies indicated that blood feeding stimulates hemocytes to increase in abundance, but how this increase in abundance is regulated is unknown. Here, we determined that phagocytic granulocytes and oenocytoids express the A. aegypti insulin receptor (AaMIR). We then showed that: 1) decapitation of mosquitoes after blood feeding inhibited hemocyte proliferation, 2) a single dose of insulin-like peptide 3 (ILP3) sufficient to stimulate egg production rescued proliferation, and 3) knockdown of the AaMIR inhibited ILP3 rescue activity. Infection studies indicated that increased hemocyte abundance enhanced clearance of the bacterium Escherichia coli at lower levels of infection. Surprisingly, however, non-blood fed females better survived intermediate and high levels of E. coli infection than blood fed females. Taken together, our results reveal a previously unrecognized role for the insulin signaling pathway in regulating hemocyte proliferation. Our results also indicate that blood feeding enhances resistance to E. coli at lower levels of infection but reduces tolerance at higher levels of infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.