ArticleNucleic acids research2012
Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Participants in Transcription-Replication Conflict and Their Role in Formation and Resolution of R-Loops.International journal of molecular sciences · 2025Review
- Transcription-replication coordination revealed in single live cells.Nucleic acids research · 2022Article
- Causes and consequences of RNA polymerase II stalling during transcript elongation.Nature reviews. Molecular cell biology · 2021Review
- Checkpoint inhibition of origin firing prevents DNA topological stress.Genes & development · 2019Article
- Transcription-mediated replication hindrance: a major driver of genome instability.Genes & development · 2019Review
- Structure and Chromosomal Organization of Yeast Genes Regulated by Topoisomerase II.International journal of molecular sciences · 2018Review
- DNA Topoisomerase Iα Affects the Floral Transition.Plant physiology · 2017Article
- Transcription-replication conflicts: how they occur and how they are resolved.Nature reviews. Molecular cell biology · 2016Review
- RNA Polymerase Collision versus DNA Structural Distortion: Twists and Turns Can Cause Break Failure.Molecular cell · 2016Review
- Dissecting the Roles of Divergent and Convergent Transcription in Chromosome Instability.Cell reports · 2016Article
- Transcription and recombination: when RNA meets DNA.Cold Spring Harbor perspectives in biology · 2014Review
- Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.The EMBO journal · 2014Article
- Supercoiling in DNA and chromatin.Current opinion in genetics & development · 2014Review
- High levels of transcription stimulate transversions at GC base pairs in yeast.Environmental and molecular mutagenesis · 2013Article
- RNA polymerase II collision interrupts convergent transcription.Molecular cell · 2012Article
- Topoisomerase II is required for the production of long Pol II gene transcripts in yeast.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the theoretical bases for the association of topoisomerases and supercoiling changes with transcription and replication, our knowledge of the impact of topological constraints on transcription and replication is incomplete. Although mutation of topoisomerases affects expression and stability of the rDNA region it is not clear whether the same is the case for RNAPII transcription and genome integrity in other regions. We developed new assays in which two convergent RNAPII-driven genes are transcribed simultaneously. Plasmid-based systems were constructed with and without a transcription terminator between the two convergent transcription units, so that the impact of transcription interference could also be evaluated. Using these assays we show that Topos I and II play roles in RNAPII transcription in vivo and reduce the stability of RNAPII-transcribed genes in Saccharomyces cerevisiae. Supercoiling accumulation in convergent transcription units impairs RNAPII transcription in top1Δ strains, but Topo II is also required for efficient transcription independent of Topo I and of detectable supercoiling accumulation. Our work shows that topological constraints negatively affect RNAPII transcription and genetic integrity, and provides an assay to study gene regulation by transcription interference.
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