ArticleBiology of reproduction2012
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) is not essential for mouse fertilization.
Article in Biology of reproduction, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- TEX29 is a novel acrosome marker dispensable for spermatogenesis and fertilization in mice.Journal of assisted reproduction and genetics · 2026Article
- Effects of Vitamin C, Rosmarinic Acid, or Quercetin on Fertilisation-Related Gene Expression in Porcine Cumulus-Oocyte Complexes During In Vitro Maturation.International journal of molecular sciences · 2026Article
- Dynamic Remodeling of the Zona Pellucida: Implications for Oocyte Competence and Assisted Reproduction.International journal of molecular sciences · 2025Review
- GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding.Nature communications · 2025Article
- Sexual selection and pseudogenization in primate fertilization.Evolution; international journal of organic evolution · 2025Article
- Functional Methods for Studying Sperm-Zona Pellucida Interaction in Mammals.Methods and protocols · 2025Review
- Review
- Acrosomal Matrix Proteins sp56 and AM67 and Gamete Adhesion in Mammals.Academia biology · 2025Article
- Recurrent Independent Pseudogenization Events of the Sperm Fertilization Gene ZP3r in Apes and Monkeys.Journal of molecular evolution · 2024Article
- Membrane potential hyperpolarization: a critical factor in acrosomal exocytosis and fertilization in sperm within the female reproductive tract.Frontiers in cell and developmental biology · 2024Article
- Molecular mechanisms and evolution of fertilization proteins.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- Review
- NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.Science (New York, N.Y.) · 2020Article
- Molecular evolution of mammalian genes with epistatic interactions in fertilization.BMC evolutionary biology · 2019Article
- Article
- Genome-wide survey in African Americans demonstrates potential epistasis of fitness in the human genome.Genetic epidemiology · 2017Article
- Gamete activation: basic knowledge and clinical applications.Human reproduction update · 2016Review
- The Acrosomal Matrix.Advances in anatomy, embryology, and cell biology · 2016Review
- PKA-dependent phosphorylation of LIMK1 and Cofilin is essential for mouse sperm acrosomal exocytosis.Developmental biology · 2015Article
- Redistribution of the intra-acrosomal EGFP before acrosomal exocytosis in mouse spermatozoa.Reproduction (Cambridge, England) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
Abstract
In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.