ArticleMolecular and cellular biochemistry2012
Cell apoptosis induced by zinc deficiency in osteoblastic MC3T3-E1 cells via a mitochondrial-mediated pathway.
Article in Molecular and cellular biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Mitochondria: a key regulator of programmed cell death in OP.Frontiers in endocrinology · 2025Review
- Hyperglycemia-induced oxidative stress exacerbates mitochondrial apoptosis damage to cochlear stria vascularis pericytes via the ROS-mediated Bcl-2/CytC/AIF pathway.Redox report : communications in free radical research · 2024Article
- The association between dietary zinc intake and osteopenia, osteoporosis in patients with rheumatoid arthritis.BMC musculoskeletal disorders · 2024Article
- The Role of Trace Elements and Minerals in Osteoporosis: A Review of Epidemiological and Laboratory Findings.Biomolecules · 2023Review
- Iron, Zinc, Copper, Cadmium, Mercury, and Bone Tissue.International journal of environmental research and public health · 2023Review
- Bioactive elements manipulate bone regeneration.Biomaterials translational · 2023Review
- Depletion of Zinc Causes Osteoblast Apoptosis with Elevation of Leptin Secretion and Phosphorylation of JAK2/STAT3.Nutrients · 2022Article
- Current Research on Zinc Oxide Nanoparticles: Synthesis, Characterization, and Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2022Review
- Biodegradable Zn-Cu-Fe Alloy as a Promising Material for Craniomaxillofacial Implants: AnFrontiers in chemistry · 2022Article
- A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells.International journal of molecular sciences · 2021Article
- Effects of Extracellular Osteoanabolic Agents on the Endogenous Response of Osteoblastic Cells.Cells · 2021Review
- Zirconia Nanoparticles Induce HeLa Cell Death Through Mitochondrial Apoptosis and Autophagy Pathways Mediated by ROS.Frontiers in chemistry · 2021Article
- Zinc as a Therapeutic Agent in Bone Regeneration.Materials (Basel, Switzerland) · 2020Review
- Uncovering the Anticancer Mechanism of Compound Sophorae Decoction against Ulcerative Colitis-Related Colorectal Cancer in Mice.Evidence-based complementary and alternative medicine : eCAM · 2019Article
- Zinc inhibits the reproductive toxicity of Zearalenone in immortalized murine ovarian granular KK-1 cells.Scientific reports · 2015Article
- Effects of SiO2, SrO, MgO, and ZnO dopants in tricalcium phosphates on osteoblastic Runx2 expression.Journal of biomedical materials research. Part A · 2014Article
- Parkinson's disease-associated human ATP13A2 (PARK9) deficiency causes zinc dyshomeostasis and mitochondrial dysfunction.Human molecular genetics · 2014Article
- The biology of zinc transport in mammary epithelial cells: implications for mammary gland development, lactation, and involution.Journal of mammary gland biology and neoplasia · 2014Review
- The role of metallothionein in oxidative stress.International journal of molecular sciences · 2013Article
- TPEN induces apoptosis independently of zinc chelator activity in a model of acute lymphoblastic leukemia and ex vivo acute leukemia cells through oxidative stress and mitochondria caspase-3- and AIF-dependent pathways.Oxidative medicine and cellular longevity · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deficiency of zinc plays an important role in the pathogenesis of osteoporosis; however, the underlying mechanism is not well understood. Apoptosis of osteoblast causing the loss of bone mass is an important event in the osteoporosis. In this article, we investigated whether zinc deficiency would induce cell apoptosis in MC3T3-E1 cells and ask if it is involved in mitochondrial-mediated pathway. Significant increased apoptosis were observed in zinc deficiency group (ZnD: 5 μM TPEN and 1 μM zinc) compared with untreated control or zinc adequacy group (ZnA: 5 μM TPEN and 15 μM zinc). The mitochondrial membrane potential was strikingly reduced in ZnD group. Furthermore, we observed that the levels of Bax in mitochondria fraction and cyto c, AIF, and cleaved caspase-3/-9 in cytosol fraction were increased in ZnD group. We proposed that zinc deficiency would induce the translocation of Bax into mitochondria, which could lead to the reduction in mitochondrial membrane potential as well as the increase in mitochondrial membrane permeability. In addition, cyto c and AIF were released from mitochondria into the cytosol, which finally activated caspase-dependent and caspase-independent apoptosis processes in MC3T3-E1 cells. Our findings suggested that zinc deficiency is capable of inducing apoptosis through a mitochondria-mediated pathway in osteoblastic cells.
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