ArticleThe Journal of biological chemistry2011
Role of amino acid insertions on intermolecular forces between arginine peptide condensed DNA helices: implications for protamine-DNA packaging in sperm.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Unlocking a Biological Interface of Chiral Supramolecular Helical Polymers.Journal of the American Chemical Society · 2025Article
- Phosphorylation of the protamine-like protein from baculovirus affects its DNA binding and virus propagation.Nucleic acids research · 2025Article
- Single-molecule measurements of double-stranded DNA condensation.Biophysical journal · 2025Review
- A DNA condensation code for linker histones.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Exploring Structures and Dynamics of Protamine Molecules through Molecular Dynamics Simulations.ACS omega · 2022Article
- Alternative direct-to-amplification sperm cell lysis techniques for sexual assault sample processing.Journal of forensic sciences · 2022Article
- Biodegradable Polymers for Gene Delivery.Molecules (Basel, Switzerland) · 2019Review
- Amino Acid Sequence of Oligopeptide Causes Marked Difference in DNA Compaction and Transcription.Biophysical journal · 2019Article
- A Comprehensive Biophysical Analysis of the Effect of DNA Binding Drugs on Protamine-induced DNA Condensation.Scientific reports · 2019Article
- The effects of IAM38 blocking or CD4 blocking on the binding of exogenous DNA in rabbit sperm.Molecular biology reports · 2019Article
- Sequence-dependent DNA condensation as a driving force of DNA phase separation.Nucleic acids research · 2018Article
- Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin.Biophysical journal · 2017Article
- Tuning DNA Condensation with Zwitterionic Polyamidoamine (zPAMAM) Dendrimers.Macromolecules · 2017Article
- Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation.Nature communications · 2016Article
- Helical structure determines different susceptibilities of dsDNA, dsRNA, and tsDNA to counterion-induced condensation.Biophysical journal · 2013Article
- A comparison of DNA compaction by arginine and lysine peptides: a physical basis for arginine rich protamines.Biochemistry · 2013Article
- Competition between supercoils and toroids in single molecule DNA condensation.Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In spermatogenesis, chromatin histones are replaced by arginine-rich protamines to densely compact DNA in sperm heads. Tight packaging is considered necessary to protect the DNA from damage. To better understand the nature of the forces condensing protamine-DNA assemblies and their dependence on amino acid content, the effect of neutral and negatively charged amino acids on DNA-DNA intermolecular forces was studied using model peptides containing six arginines. We have previously observed that the neutral amino acids in salmon protamine decrease the net attraction between protamine-DNA helices compared with the equivalent homo-arginine peptide. Using osmotic stress coupled with x-ray scattering, we have investigated the component attractive and repulsive forces that determine the net attraction and equilibrium interhelical distance as a function of the chemistry, position, and number of the amino acid inserted. Neutral amino acids inserted into hexa-arginine increase the short range repulsion while only slightly affecting longer range attraction. The amino acid content alone of salmon protamine is enough to rationalize the forces that package DNA in sperm heads. Inserting a negatively charged amino acid into hexa-arginine dramatically weakens the net attraction. Both of these observations have biological implications for protamine-DNA packaging in sperm heads.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.