ArticleThe Journal of biological chemistry2011
MAPKKK-independent regulation of the Hog1 stress-activated protein kinase in Candida albicans.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- A roadmap to chemically reactive species: how diverse oxidants affectMicrobiology and molecular biology reviews : MMBR · 2025Review
- Synthesis and Antimicrobial Evaluation of Chroman-4-One and Homoisoflavonoid Derivatives.Molecules (Basel, Switzerland) · 2025Article
- Stress contingent changes in Hog1 pathway architecture and regulation in Candida albicans.PLoS pathogens · 2024Article
- Synthesis of Fully Deacetylated Quaternized Chitosan with Enhanced Antimicrobial Activity and Low Cytotoxicity.Antibiotics (Basel, Switzerland) · 2022Article
- Kinase Hog1 and Adr1 Opposingly Regulate Haploid Cell Morphology by Controlling Vacuole Size inJournal of fungi (Basel, Switzerland) · 2022Article
- Review
- The impact of the Fungus-Host-Microbiota interplay upon Candida albicans infections: current knowledge and new perspectives.FEMS microbiology reviews · 2021Review
- Immune cells fold and damage fungal hyphae.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Adaptation to Endoplasmic Reticulum Stress inFrontiers in microbiology · 2021Article
- Protein-Protein Interactions inFrontiers in microbiology · 2019Review
- Stress-Activated Protein Kinases in Human Fungal Pathogens.Frontiers in cellular and infection microbiology · 2019Review
- Candida albicans Ras1 Inactivation Increases Resistance to Phagosomal Killing by Human Neutrophils.Infection and immunity · 2018Article
- Article
- Article
- Non-canonical Activities of Hog1 Control Sensitivity ofFrontiers in cellular and infection microbiology · 2018Article
- PbsB Regulates Morphogenesis, Aflatoxin B1 Biosynthesis, and Pathogenicity ofFrontiers in cellular and infection microbiology · 2018Article
- Stress-induced nuclear accumulation is dispensable for Hog1-dependent gene expression and virulence in a fungal pathogen.Scientific reports · 2017Article
- Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity.Molecular plant pathology · 2017Article
- Blocking two-component signalling enhances Candida albicans virulence and reveals adaptive mechanisms that counteract sustained SAPK activation.PLoS pathogens · 2017Article
- Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes.Current microbiology · 2016Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The Hog1 stress-activated protein kinase regulates both stress responses and morphogenesis in Candida albicans and is essential for the virulence of this major human pathogen. Stress-induced Hog1 phosphorylation is regulated by the upstream MAPKK, Pbs2, which in turn is regulated by the MAPKKK, Ssk2. Here, we have investigated the role of phosphorylation of Hog1 and Pbs2 in Hog1-mediated processes in C. albicans. Mutation of the consensus regulatory phosphorylation sites of Hog1 (Thr-174/Tyr-176) and Pbs2 (Ser-355/Thr-359), to nonphosphorylatable residues, resulted in strains that phenocopied hog1Δ and pbs2Δ cells. Consistent with this, stress-induced phosphorylation of Hog1 was abolished in cells expressing nonphosphorylatable Pbs2 (Pbs2(AA)). However, mutation of the consensus sites of Pbs2 to phosphomimetic residues (Pbs2(DD)) failed to constitutively activate Hog1. Furthermore, Ssk2-independent stress-induced Hog1 activation was observed in Pbs2(DD) cells. Collectively, these data reveal a previously uncharacterized MAPKKK-independent mechanism of Hog1 activation in response to stress. Although Pbs2(DD) cells did not exhibit high basal levels of Hog1 phosphorylation, overexpression of an N-terminal truncated form of Ssk2 did result in constitutive Hog1 activation, which was further increased upon stress. Significantly, both Pbs2(AA) and Pbs2(DD) cells displayed impaired stress resistance and attenuated virulence in a mouse model of disease, whereas only Pbs2(AA) cells exhibited the morphological defects associated with loss of Hog1 function. This indicates that Hog1 mediates C. albicans virulence by conferring stress resistance rather than regulating morphogenesis.
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