ArticleViruses2009
An economical tandem multiplex real-time PCR technique for the detection of a comprehensive range of respiratory pathogens.
Article in Viruses, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed.
- High fractional exhaled nitric oxide and sputum eosinophils are associated with an increased risk of future virus-induced exacerbations: A prospective cohort study.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2017Trial
- Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016-2020.Virologica Sinica · 2025Article
- Observational Study on the Clinical Reality of Community-Acquired Respiratory Virus Infections in Adults and Older Individuals.Pathogens (Basel, Switzerland) · 2024Observational
- Impact of the Omicron Strain on Febrile Convulsions Requiring Hospitalization in Children: A Single-Center Observational Study.Pediatric reports · 2024Article
- Article
- LPS binding protein and activation signatures are upregulated during asthma exacerbations in children.Respiratory research · 2023Article
- The seasonality of respiratory syncytial virus in Western Australia prior to implementation of SARS-CoV-2 non-pharmaceutical interventions.Influenza and other respiratory viruses · 2023Article
- Off-season RSV epidemics in Australia after easing of COVID-19 restrictions.Nature communications · 2022Article
- Personal Network Inference Unveils Heterogeneous Immune Response Patterns to Viral Infection in Children with Acute Wheezing.Journal of personalized medicine · 2021Article
- Incidence, Etiology, and Environmental Risk Factors of Community-Acquired Pneumonia Requiring Hospitalization in China: A 3-Year, Prospective, Age-Stratified, Multicenter Case-Control Study.Open forum infectious diseases · 2021Article
- Characteristics and etiology of hospitalized pediatric community-acquired pneumonia in Taiwan.Journal of the Formosan Medical Association = Taiwan yi zhi · 2020Article
- Nasopharyngeal density of respiratory viruses in childhood pneumonia in a highly vaccinated setting: findings from a case-control study.BMJ open respiratory research · 2020Article
- Increased Detection of Viruses in Children with Respiratory Tract Infection Using PCR.International journal of environmental research and public health · 2020Article
- Post-pandemic influenza A/H1N1pdm09 is associated with more severe outcomes than A/H3N2 and other respiratory viruses in adult hospitalisations.Epidemiology and infection · 2019Article
- Nasal Cytokine Profiles of Patients Hospitalised with Respiratory Wheeze Associated with Rhinovirus C.Viruses · 2019Article
- The impact of influenza infection on young children, their family and the health care system.Influenza and other respiratory viruses · 2019Article
- Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children.PloS one · 2019Article
- Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma.Respiratory medicine · 2018Article
- Role of viral and bacterial pathogens in causing pneumonia among Western Australian children: a case-control study protocol.BMJ open · 2018Article
- A microbiome case-control study of recurrent acute otitis media identified potentially protective bacterial genera.BMC microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumovirus and picornavirus PCR assays were required to complete the acute respiratory pathogen profile. Potential pathogens were detected in 85 (70%) of pernasal aspirates collected from 121 children with acute respiratory symptoms. Multiple pathogens were detected in 29 (24%) of those samples. The tandem multiplex real-time PCR was an efficient method for the rapid detection of multiple pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.