ArticleJournal of virology2011
VPg-primed RNA synthesis of norovirus RNA-dependent RNA polymerases by using a novel cell-based assay.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 87 citations in OpenAlex.
- Drivers of the paradigm shift in norovirus diagnostics: technological innovation, contextual demands, and collaborative synergy.Virology journal · 2026Review
- Engineered VPg saRNA achieves cap-independent, low-immunogenic and precise encoding of therapeutic proteins in vivo.Nature communications · 2026Article
- BTP2 restricts Tulane virus and human norovirus replication independent of store-operated calcium entry.Journal of virology · 2025Article
- Genomic characterization of norovirus and Sapovirus from a diarrhea outbreak in a school linked to heavy rainfall.Frontiers in microbiology · 2025Article
- Out-of-sync evolutionary patterns and mutual interplay of major and minor capsid proteins in norovirus GII.2.The Journal of general virology · 2024Article
- Highs and Lows in Calicivirus Reverse Genetics.Viruses · 2024Review
- Atypical activation of the RNA sensor MDA5 by hepatitis C virus.The FEBS journal · 2024Article
- Review
- Evolutionary analyses of emerging GII.2[P16] and GII.4 Sydney [P16] noroviruses.Virus evolution · 2022Article
- Interferon responses to norovirus infections: current and future perspectives.The Journal of general virology · 2021Review
- Discovery of a Novel Class of Norovirus Inhibitors with High Barrier of Resistance.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Generation of Nucleic Acid Aptamer Candidates against a Novel Calicivirus Protein Target.Viruses · 2021Article
- Protein Nucleotidylylation in +ssRNA Viruses.Viruses · 2021Review
- Human Norovirus NTPase Antagonizes Interferon-β Production by Interacting With IkB Kinase ε.Frontiers in microbiology · 2021Article
- Article
- Guanylate-binding protein 2 orchestrates innate immune responses against murine norovirus and is antagonized by the viral protein NS7.The Journal of biological chemistry · 2020Article
- Quantitation of norovirus-specific IgG before and after infection in immunocompromised patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Human Norovirus Proteins: Implications in the Replicative Cycle, Pathogenesis, and the Host Immune Response.Frontiers in immunology · 2020Review
- Article
- Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity.The Journal of biological chemistry · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Molecular studies of human noroviruses (NoV) have been hampered by the lack of a permissive cell culture system. We have developed a sensitive and reliable mammalian cell-based assay for the human NoV GII.4 strain RNA-dependent RNA polymerase (RdRp). The assay is based on the finding that RNAs synthesized by transiently expressed RdRp can stimulate retinoic acid-inducible gene I (RIG-I)-dependent reporter luciferase production via the beta interferon promoter. Comparable activities were observed for the murine norovirus (MNV) RdRp. RdRps with mutations at divalent metal ion binding residues did not activate RIG-I signaling. Furthermore, both NoV and MNV RdRp activities were stimulated by the coexpression of their respective VPg proteins, while mutations in the putative site of nucleotide linkage on VPg abolished most of their stimulatory effects. Sequencing of the RNAs linked to VPg revealed that the cellular trans-Golgi network protein 2 (TGOLN2) mRNA was the template for VPg-primed RNA synthesis. Small interfering RNA knockdown of RNase L abolished the enhancement of signaling that occurred in the presence of VPg. Finally, the coexpression of each of the other NoV proteins revealed that p48 (also known as NS1-2) and VP1 enhanced and that VP2 reduced the RdRp activity. The assay should be useful for the dissection of the requirements for NoV RNA synthesis as well as the identification of inhibitors of the NoV RdRp.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.