ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
A signaling mechanism coupling netrin-1/deleted in colorectal cancer chemoattraction to SNARE-mediated exocytosis in axonal growth cones.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Ablation of CD38 in multiple myeloma cells leads to an aggressive phenotype in a mouse xenograft model.Blood advances · 2026Article
- Syntaxin-1 is necessary for UNC5A-C/Netrin-1-dependent macropinocytosis and chemorepulsion.Frontiers in molecular neuroscience · 2023Article
- Expression of the schizophrenia associated geneFrontiers in neuroscience · 2023Article
- SNARE Proteins in Synaptic Vesicle Fusion.Advances in neurobiology · 2023Article
- The importance of fasciculation and elongation protein zeta-1 in neural circuit establishment and neurological disorders.Neural regeneration research · 2022Review
- Growth cone repulsion to Netrin-1 depends on lipid raft microdomains enriched in UNC5 receptors.Cellular and molecular life sciences : CMLS · 2021Article
- Nystatin Regulates Axonal Extension and Regeneration by Modifying the Levels of Nitric Oxide.Frontiers in molecular neuroscience · 2020Article
- Ephrin-A5 potentiates netrin-1 axon guidance by enhancing Neogenin availability.Scientific reports · 2019Article
- Axon guidance pathway genes are associated with schizophrenia risk.Experimental and therapeutic medicine · 2018Article
- Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.Oncotarget · 2018Article
- A conserved role for Syntaxin-1 in pre- and post-commissural midline axonal guidance in fly, chick, and mouse.PLoS genetics · 2018Article
- SNARE complex in axonal guidance and neuroregeneration.Neural regeneration research · 2018Review
- Revisiting Netrin-1: One Who Guides (Axons).Frontiers in cellular neuroscience · 2018Review
- Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants.Autophagy · 2017Article
- Lentiviral-Mediated Netrin-1 Overexpression Improves Motor and Sensory Functions in SCT Rats Associated with SYP and GAP-43 Expressions.Molecular neurobiology · 2017Article
- Where does axon guidance lead us?F1000Research · 2017Review
- Beyond the cytoskeleton: The emerging role of organelles and membrane remodeling in the regulation of axon collateral branches.Developmental neurobiology · 2016Review
- Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- Differential expression of neurogenes among breast cancer subtypes identifies high risk patients.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed cell migration and axonal guidance are essential steps in neural development. Both processes are controlled by specific guidance cues that activate the signaling cascades that ultimately control cytoskeletal dynamics. Another essential step in migration and axonal guidance is the regulation of plasmalemma turnover and exocytosis in leading edges and growth cones. However, the cross talk mechanisms linking guidance receptors and membrane exocytosis are not understood. Netrin-1 is a chemoattractive cue required for the formation of commissural pathways. Here, we show that the Netrin-1 receptor deleted in colorectal cancer (DCC) forms a protein complex with the t-SNARE (target SNARE) protein Syntaxin-1 (Sytx1). This interaction is Netrin-1 dependent both in vitro and in vivo, and requires specific Sytx1 and DCC domains. Blockade of Sytx1 function by using botulinum toxins abolished Netrin-1-dependent chemoattraction of axons in mouse neuronal cultures. Similar loss-of-function experiments in the chicken spinal cord in vivo using dominant-negative Sytx1 constructs or RNAi led to defects in commissural axon pathfinding reminiscent to those described in Netrin-1 and DCC loss-of-function models. We also show that Netrin-1 elicits exocytosis at growth cones in a Sytx1-dependent manner. Moreover, we demonstrate that the Sytx1/DCC complex associates with the v-SNARE (vesicle SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) and that knockdown of TI-VAMP in the commissural pathway in the spinal cord results in aberrant axonal guidance phenotypes. Our data provide evidence of a new signaling mechanism that couples chemotropic Netrin-1/DCC axonal guidance and Sytx1/TI-VAMP SNARE proteins regulating membrane turnover and exocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.