Evidence map›Paper›PMID 21994333›Full record

ArticleGut2012

Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer.

James J Farrell, Lei Zhang, Hui Zhou, David Chia, David Elashoff, David Akin, Bruce J Paster, Kaumudi Joshipura, David T W Wong

2 registry-linked trialsOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gut, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 392 papers, 7 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
392citing papers in PubMed, 7 pooled it
13.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04523987 phase1active not recruitingnot on this mapstarted 2019, after this paper: background citation

A Pilot Study of Ciprofloxacin Plus Gemcitabine and Nab-Paclitaxel Chemotherapy in Patients With Metastatic Pancreatic Ductal Adenocarcinoma.

TypeinterventionalSponsorNational University Hospital, SingaporeRan2019 to 2027Enrolled10ConditionsMetastatic Pancreatic Ductal AdenocarcinomaArmsCiprofloxacin
NCT04993846 unknown statusnot on this mapstarted 2021, after this paper: background citation

Pancreatic canceR pErioDontAl sTatus and Oral micRobiome - PREDATOR Study

TypeobservationalSponsorAzienda Ospedaliera Universitaria Integrata VeronaRan2021 to 2023Enrolled120ConditionsOral Microbiome, Pancreatic Cancer, IPMN, ParodontopathyArmsDental plaque sampling, qPCR
3 · Its place in the literature

Who cites it

392 citing papers in PubMed, 7 syntheses or guidelines pooled it, 652 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Pooled it
  5. Pooled it
  6. Pooled it
  7. Pooled it
  8. Trial
  9. Alterations in the Duodenal Fluid Microbiome of Patients With Pancreatic Cancer.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2022
    Trial
  10. Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Review
  19. Review
  20. Review

332 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

James J FarrellDivision of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. jfarrell@mednet.ucla.edu
Lei Zhang
Hui Zhou
David Chia
David Elashoff
David Akin
Bruce J Paster
Kaumudi Joshipura
David T W Wong
University of California, Los Angeles · USSamueli Institute · USUniversity of Puerto Rico System · PR

Funding

Salivary Biomarkers for Early Oral Cancer DetectionR01DE017170 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WONG, DAVID T · 2008 to 2011
$1.5M
Clinical Research and Mentoring in Oral EpidemiologyK24DE016884 · NIDCR · UNIVERSITY OF PUERTO RICO MED SCIENCES · PI JOSHIPURA, KAUMUDI J · 2005 to 2009
$758k
Collection, Stabilization and Storage of Saliva Samples for Cancer ResearchR21CA126733 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WONG, DAVID T · 2008 to 2009
$381k
NCI NIH HHS R21 CA126733NCI NIH HHS R21CA126733NIDCR NIH HHS K24 DE016884NIDCR NIH HHS R01 DE017170NIDCR NIH HHS R01DE017170
6 · The paper itself

Abstract

objectiveThe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. In this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.

methodsThis study was divided into three phases: (1) microbial profiling using the Human Oral Microbe Identification Microarray to investigate salivary microbiota variation between 10 resectable patients with pancreatic cancer and 10 matched healthy controls, (2) identification and verification of bacterial candidates by real-time quantitative PCR (qPCR) and (3) validation of bacterial candidates by qPCR on an independent cohort of 28 resectable pancreatic cancer, 28 matched healthy control and 27 chronic pancreatitis samples.

resultsComprehensive comparison of the salivary microbiota between patients with pancreatic cancer and healthy control subjects revealed a significant variation of salivary microflora. Thirty-one bacterial species/clusters were increased in the saliva of patients with pancreatic cancer (n=10) in comparison to those of the healthy controls (n=10), whereas 25 bacterial species/clusters were decreased. Two out of six bacterial candidates (Neisseria elongata and Streptococcus mitis) were validated using the independent samples, showing significant variation (p<0.05, qPCR) between patients with pancreatic cancer and controls (n=56). Additionally, two bacteria (Granulicatella adiacens and S mitis) showed significant variation (p<0.05, qPCR) between chronic pancreatitis samples and controls (n=55). The combination of two bacterial biomarkers (N elongata and S mitis) yielded a receiver operating characteristic plot area under the curve value of 0.90 (95% CI 0.78 to 0.96, p<0.0001) with a 96.4% sensitivity and 82.1% specificity in distinguishing patients with pancreatic cancer from healthy subjects.

conclusionsThe authors observed associations between variations of patients' salivary microbiota with pancreatic cancer and chronic pancreatitis. This report also provides proof of salivary microbiota as an informative source for discovering non-invasive biomarkers of systemic diseases.

Indexed as

MetagenomeAgedAged, 80 and overBacteriaBacterial Typing TechniquesBiomarkersCase-Control StudiesFemaleHumansMaleMiddle AgedPancreatic DiseasesPancreatic NeoplasmsPancreatitis, ChronicPhylogenyReal-Time Polymerase Chain ReactionBiomarkersRNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID21994333
PMCPMC3705763
OpenAlexW2115865473

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.