ArticleAmerican journal of physiology. Renal physiology2012
Do β3-adrenergic receptors play a role in guinea pig detrusor smooth muscle excitability and contractility?
Article in American journal of physiology. Renal physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Regulation of P2X1 receptors by modulators of the cAMP effectors PKA and EPAC.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Urinary bladder smooth muscle ion channels: expression, function, and regulation in health and disease.American journal of physiology. Renal physiology · 2020Review
- Properties of single-channel and whole cell ClAmerican journal of physiology. Cell physiology · 2019Article
- Central role of the BK channel in urinary bladder smooth muscle physiology and pathophysiology.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Review
- Purinergic inhibitory regulation of murine detrusor muscles mediated by PDGFRα+ interstitial cells.The Journal of physiology · 2014Article
- Functional BK channels facilitate the β3-adrenoceptor agonist-mediated relaxation of nerve-evoked contractions in rat urinary bladder smooth muscle isolated strips.European journal of pharmacology · 2013Article
- Single-channel biophysical and pharmacological characterizations of native human large-conductance calcium-activated potassium channels in freshly isolated detrusor smooth muscle cells.Pflugers Archiv : European journal of physiology · 2013Article
- NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels.British journal of pharmacology · 2013Article
- Novel role for the transient potential receptor melastatin 4 channel in guinea pig detrusor smooth muscle physiology.American journal of physiology. Cell physiology · 2013Article
- Article
- Selective inhibition of phosphodiesterase 1 relaxes urinary bladder smooth muscle: role for ryanodine receptor-mediated BK channel activation.American journal of physiology. Cell physiology · 2012Article
- SK but not IK channels regulate human detrusor smooth muscle spontaneous and nerve-evoked contractions.American journal of physiology. Renal physiology · 2012Article
- Expression and function of K(V)2-containing channels in human urinary bladder smooth muscle.American journal of physiology. Cell physiology · 2012Article
- Inhibition of phosphodiesterases relaxes detrusor smooth muscle via activation of the large-conductance voltage- and Ca²⁺-activated K⁺ channel.American journal of physiology. Cell physiology · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In many species, β3-adrenergic receptors (β3-ARs) have been reported to play a primary role in pharmacologically induced detrusor smooth muscle (DSM) relaxation. However, their role in guinea pig DSM remains controversial. The aim of this study was to investigate whether β3-ARs are expressed in guinea pig DSM and to evaluate how BRL37344 and L-755,507, two selective β3-AR agonists, modulate guinea pig DSM excitability and contractility. We used a combined experimental approach including RT-PCR, patch-clamp electrophysiology, and isometric DSM tension recordings. β3-AR mRNA message was detected in freshly isolated guinea pig DSM single cells. BRL37344 but not L-755,507 caused a slight decrease in DSM spontaneous phasic contraction amplitude and frequency in a concentration-dependent manner. In the presence of atropine (1 μM), only the spontaneous phasic contractions frequency was inhibited by BRL37344 at higher concentrations. Both BRL37344 and L-755,507 significantly decreased DSM carbachol-induced phasic and tonic contractions in a concentration-dependent manner. However, only BRL37344 inhibitory effect was partially antagonized by SR59230A (10 μM), a β3-AR antagonist. In the presence of atropine, BRL37344 and L-755,507 had no inhibitory effect on electrical field stimulation-induced contractions. Patch-clamp experiments showed that BRL37344 (100 μM) did not affect the DSM cell resting membrane potential and K(+) conductance. Although β3-ARs are expressed at the mRNA level, they play a minor to no role in guinea pig DSM spontaneous contractility without affecting cell excitability. However, BRL37344 and L-755,507 have pronounced inhibitory effects on guinea pig DSM carbachol-induced contractions. The study outlines important DSM β3-ARs species differences.
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