ArticleMethods in molecular biology (Clifton, N.J.)2012
Techniques to measure pilus retraction forces.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Visualization of Type IV Pili: Linking Structural Architecture, Dynamic Function, and Translational Opportunities.Biology · 2026Review
- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.Molecular biology of the cell · 2021Article
- Mechanomicrobiology: how bacteria sense and respond to forces.Nature reviews. Microbiology · 2020Review
- A bifunctional ATPase drives tad pilus extension and retraction.Science advances · 2019Article
- PilT and PilU are homohexameric ATPases that coordinate to retract type IVa pili.PLoS genetics · 2019Article
- Quantifying cellular forces and biomechanical properties by correlative micropillar traction force and Brillouin microscopy.Biomedical optics express · 2019Article
- Obstruction of pilus retraction stimulates bacterial surface sensing.Science (New York, N.Y.) · 2017Article
- Quantifying forces in cell biology.Nature cell biology · 2017Review
- The Vibrio cholerae Minor Pilin TcpB Initiates Assembly and Retraction of the Toxin-Coregulated Pilus.PLoS pathogens · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The importance of physical forces in biology is becoming more appreciated. Neisseria gonorrhoeaehas become a paradigm for the study of physical forces in the bacterial world. Cycles of elongations and retractions of Type IV pili enables N. gonorrhoeaebacteria to exert forces on its environment, forces that play major roles in the life cycle of this pathogen. In order to better understand the role of these forces, there is a need to fully characterize them. Here, we present two different techniques, optical tweezers and Polyacrylamide MicroPillars (PoMPs), for measuring pilus retraction forces. Initially designed for N. gonorrhoeae, these assays can be readily modified to study other pilus-bearing bacteria including Neisseria meningitidis.
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