ArticleEuropean archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry2011
Short communication: polymicrobial community in teeth associated with severe early-childhood caries.
Article in European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
aimThis was to characterise the microbial diversity in the complex dental plaque of children with severe early-childhood caries (S-ECC), using the denaturing gel gradient electrophoresis (DGGE) technique.
methodsThe DGGE technique was used as a diagnostic tool to analyse samples from the oral cavity of a patient with S-ECC. Dental plaque samples from a 3-year-old child with S-ECC were taken from the primary central maxillary incisor (biofilm on vestibular surface), primary maxillary molar (biofilm on vestibular surface), primary central maxillary incisor (dentine), primary maxillary molar (dentine) and saliva and then analysed by PCR-DGGE.
resultsThree bands occurred in all samples, moreover, 86% of similarity was observed in the pattern of bands between incisor and molar teeth biofilm samples, including four similar bands.
conclusionDGGE is a valuable tool for differentiating the microbial composition of the oral plaque in S-ECC children.
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