ArticleTissue engineering. Part A2012
Toward an in vitro vasculature: differentiation of mesenchymal stromal cells within an endothelial cell-seeded modular construct in a microfluidic flow chamber.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Bone marrow vasculature advancedFrontiers in cardiovascular medicine · 2023Review
- An Easy-to-Fabricate Microfluidic Shallow Trench Induced Three-Dimensional Cell Culturing and Imaging (STICI3D) Platform.ACS omega · 2022Article
- Endothelial Cell Distribution After Flow Exposure With Two Stent Struts Placed in Different Angles.Frontiers in physiology · 2021Article
- Bioengineering the Bone Marrow Vascular Niche.Frontiers in cell and developmental biology · 2021Review
- Engineering transferrable microvascular meshes for subcutaneous islet transplantation.Nature communications · 2019Article
- Engineering in-vitro stem cell-based vascularized bone models for drug screening and predictive toxicology.Stem cell research & therapy · 2018Review
- A novel high-speed production process to create modular components for the bottom-up assembly of large-scale tissue-engineered constructs.Advanced healthcare materials · 2015Article
- Vascular targeting of nanocarriers: perplexing aspects of the seemingly straightforward paradigm.ACS nano · 2014Review
- Del-1 overexpression in endothelial cells increases vascular density in tissue-engineered implants containing endothelial cells and adipose-derived mesenchymal stromal cells.Tissue engineering. Part A · 2014Article
- Bioreactors to influence stem cell fate: augmentation of mesenchymal stem cell signaling pathways via dynamic culture systems.Biochimica et biophysica acta · 2013Review
- Cotransplantation of adipose-derived mesenchymal stromal cells and endothelial cells in a modular construct drives vascularization in SCID/bg mice.Tissue engineering. Part A · 2012Article
- Bone marrow-derived mesenchymal stromal cells enhance chimeric vessel development driven by endothelial cell-coated microtissues.Tissue engineering. Part A · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An in vitro tissue construct amenable to perfusion was formed by randomly packing mesenchymal stromal cell (MSC)-embedded, endothelial cell (EC)-coated collagen cylinders (modules) into a microfluidic chamber. The interstices created by the random packing of the submillimeter-sized modules created EC-lined channels. Flow caused a greater than expected amount of contraction and remodeling in the modular constructs. Flow influenced the MSC to develop smooth muscle cell markers (smooth muscle actin-positive, desmin-positive, and von Willebrand factor-negative) and migrate toward the surface of the modules. When modules were coated with EC, the extent of MSC differentiation and migration increased, suggesting that the MSC were becoming smooth muscle cell- or pericyte-like in their location and phenotype. The MSC also proliferated, resulting in a substantial increase in the number of differentiated MSC. These effects were markedly less for static controls not experiencing flow. As the MSC migrated, they created new matrix that included the deposition of proteoglycans. Collectively, these results suggest that MSC-embedded modules may be useful for the formation of functional vasculature in tissue engineered constructs. Moreover, these flow-conditioned tissue engineered constructs may be of interest as three-dimensional cell-laden platforms for drug testing and biological assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.