ArticleBiomedical optics express2011
Effects of incomplete decay in fluorescence lifetime estimation.
Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Fluorescence lifetime imaging microscopy of endogenous fluorophores in health and disease.Journal of muscle research and cell motility · 2025Review
- NAD(P)H binding configurations revealed by time-resolved fluorescence and two-photon absorption.Biophysical journal · 2023Article
- Label-Free Metabolic Imaging In Vivo by Two-Photon Fluorescence Lifetime Endomicroscopy.ACS photonics · 2022Article
- Article
- Unsupervised clustering of multiparametric fluorescent images extends the spectrum of detectable cell membrane phases with sub-micrometric resolution.Biomedical optics express · 2020Article
- PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells.Biophysical journal · 2020Article
- Merger of dynamic two-photon and phosphorescence lifetime microscopy reveals dependence of lymphocyte motility on oxygen in solid and hematological tumors.Journal for immunotherapy of cancer · 2019Article
- Polystyrene Oxygen Optodes Doped with Ir(III) and Pd(II) meso-Tetrakis(pentafluorophenyl)porphyrin Using an LED-Based High-Sensitivity Phosphorimeter.Sensors (Basel, Switzerland) · 2018Article
- Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of exogenous fluorophores in mice.Biomedical optics express · 2017Article
- Article
- Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells.Nature communications · 2015Article
- Singlet-triplet annihilation in single LHCII complexes.Physical chemistry chemical physics : PCCP · 2015Article
- FLIMX: A Software Package to Determine and Analyze the Fluorescence Lifetime in Time-Resolved Fluorescence Data from the Human Eye.PloS one · 2015Article
- Testing fluorescence lifetime standards using two-photon excitation and time-domain instrumentation: rhodamine B, coumarin 6 and lucifer yellow.Journal of fluorescence · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.