Evidence map›Paper›PMID 21991541›Full record

ArticleBiomedical optics express2011

CARS microscopy for the visualization of micrometer-sized iron oxide MRI contrast agents in living cells.

Gianluca Rago, Carolin M Langer, Christian Brackman, James P R Day, Katrin F Domke, Nathanael Raschzok, Christian Schmidt, Igor M Sauer, Annika Enejder, Martina T Mogl and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Gianluca Rago
Carolin M Langer
Christian Brackman
James P R Day
Katrin F Domke
Nathanael Raschzok
Christian Schmidt
Igor M Sauer
Annika Enejder
Martina T Mogl
Mischa Bonn

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Micrometer-sized iron oxide particles (MPIOs) attract increasing interest as contrast agents for cellular tracking by clinical Magnetic Resonance Imaging (MRI). Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level. Here, we show that coherent anti-Stokes Raman scattering (CARS) microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions. CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.

Indexed as

(020.4180) Multiphoton processes(160.4236) Nanomaterials(170.3880) Medical and biological imaging(180.6900) Three-dimensional microscopy(190.4380) Nonlinear optics, four-wave mixing(300.6230) Spectroscopy, coherent anti-Stokes Raman scattering

Identifiers

PMID21991541
PMCPMC3184857

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.