ArticlePLoS neglected tropical diseases2011
Imaginal discs--a new source of chromosomes for genome mapping of the yellow fever mosquito Aedes aegypti.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Chromosomal rearrangements in mosquitoes: from micro- to macroevolution.Current opinion in insect science · 2025Review
- Chromosomal Inversions and Their Potential Impact on the Evolution of Arboviral Vector Aedes aegypti.Genome biology and evolution · 2025Article
- The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes.BMC biology · 2024Article
- Article
- Improved reference genome of the arboviral vector Aedes albopictus.Genome biology · 2020Article
- Chromatin Structure and Function in Mosquitoes.Frontiers in genetics · 2020Review
- The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor.Parasites & vectors · 2018Article
- Article
- Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements.PLoS neglected tropical diseases · 2016Article
- A standard cytogenetic map of Culex quinquefasciatus polytene chromosomes in application for fine-scale physical mapping.Parasites & vectors · 2015Article
- Article
- Genomic composition and evolution of Aedes aegypti chromosomes revealed by the analysis of physically mapped supercontigs.BMC biology · 2014Article
- Insights into the preservation of the homomorphic sex-determining chromosome of Aedes aegypti from the discovery of a male-biased gene tightly linked to the M-locus.Genome biology and evolution · 2014Article
- An integrated linkage, chromosome, and genome map for the yellow fever mosquito Aedes aegypti.PLoS neglected tropical diseases · 2013Article
- Fluorescent in situ hybridization on mitotic chromosomes of mosquitoes.Journal of visualized experiments : JoVE · 2012Article
- High-throughput physical mapping of chromosomes using automated in situ hybridization.Journal of visualized experiments : JoVE · 2012Article
- Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti.The Journal of heredityArticle
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6 authors.
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Abstract
backgroundThe mosquito Aedes aegypti is the primary global vector for dengue and yellow fever viruses. Sequencing of the Ae. aegypti genome has stimulated research in vector biology and insect genomics. However, the current genome assembly is highly fragmented with only ~31% of the genome being assigned to chromosomes. A lack of a reliable source of chromosomes for physical mapping has been a major impediment to improving the genome assembly of Ae. aegypti. METHODOLOGY/PRINCIPAL
findingsIn this study we demonstrate the utility of mitotic chromosomes from imaginal discs of 4(th) instar larva for cytogenetic studies of Ae. aegypti. High numbers of mitotic divisions on each slide preparation, large sizes, and reproducible banding patterns of the individual chromosomes simplify cytogenetic procedures. Based on the banding structure of the chromosomes, we have developed idiograms for each of the three Ae. aegypti chromosomes and placed 10 BAC clones and a 18S rDNA probe to precise chromosomal positions.
conclusionThe study identified imaginal discs of 4(th) instar larva as a superior source of mitotic chromosomes for Ae. aegypti. The proposed approach allows precise mapping of DNA probes to the chromosomal positions and can be utilized for obtaining a high-quality genome assembly of the yellow fever mosquito.
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