ArticlePloS one2011
Agrobacterium rhizogenes-mediated transformation of the parasitic plant Phtheirospermum japonicum.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 99 citations in OpenAlex.
- Interplant signal transduction between dodder (Cuscuta) and their hosts.Plant & cell physiology · 2026Review
- Parasitic Plant-Host Interactions: Molecular Mechanisms and Agricultural Resistance Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Integration of single nucleus RNA-seq and bulk RNA-seq reveals gene regulatory networks for vascular connection between parasitic plants and host plants.Journal of plant research · 2025Article
- Neofunctionalized RGF pathways drive haustorial organogenesis in parasitic plants.Science advances · 2025Article
- Effects of Exogenous Phenolic Acids on Haustorium Induction ofInternational journal of molecular sciences · 2025Article
- A long-distance inhibitory system regulates haustoria numbers in parasitic plants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Agrobacterium-mediated Cuscuta campestris transformation as a tool for understanding plant-plant interactions.The New phytologist · 2025Article
- An efficient Rhizobium rhizogenes-mediated transformation system for Cuscuta campestris.PloS one · 2025Article
- Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Article
- What we know so far and what we can expect next: A molecular investigation of plant parasitism.Genetics and molecular biology · 2024Article
- Pectin modifications promote haustoria development in the parasitic plant Phtheirospermum japonicum.Plant physiology · 2023Article
- A roadmap of haustorium morphogenesis in parasitic plants.Journal of experimental botany · 2023Article
- Role of Strigolactones in the Host Specificity of Broomrapes and Witchweeds.Plant & cell physiology · 2023Review
- A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes.PloS one · 2023Article
- Strigol induces germination of the facultative parasitic plantPlant signaling & behavior · 2022Article
- Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants.Nature communications · 2022Article
- Nitrogen represses haustoria formation through abscisic acid in the parasitic plant Phtheirospermum japonicum.Nature communications · 2022Article
- Genomic reconfiguration in parasitic plants involves considerable gene losses alongside global genome size inflation and gene births.Plant physiology · 2021Article
- An Optimized Transformation System and Functional Test ofInternational journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL
findingsWe developed transient and stable transformation systems for Phtheirospermum japonicum, a facultative parasitic plant in the Orobanchaceae. The transformation protocol was established by a combination of sonication and acetosyringone treatments using the hairy-root-inducing bacterium, Agrobacterium rhizogenes and young seedlings. Transgenic hairy roots of P. japonicum were obtained from cotyledons 2 to 3 weeks after A. rhizogenes inoculation. The presence and the expression of transgenes in P. japonicum were verified by genomic PCR, Southern blot and RT-PCR methods. Transgenic roots derived from A. rhizogenes-mediated transformation were able to develop haustoria on rice and maize roots. Transgenic roots also formed apparently competent haustoria in response to 2,6-dimethoxy-1,4-benzoquinone (DMBQ), a haustorium-inducing chemical. Using this system, we introduced a reporter gene with a Cyclin B1 promoter into P. japonicum, and visualized cell division during haustorium formation.
conclusionsWe provide an easy and efficient method for hairy-root transformation of P. japonicum. Transgenic marker analysis revealed that cell divisions during haustorium development occur 24 h after DMBQ treatment. The protocols described here will allow functional analysis of genes involved in plant parasitism.
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