ArticleJournal of translational medicine2011
Multiplex serum biomarker assessments: technical and biostatistical issues.
Article in Journal of translational medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Improved prognosis and evidence of enhanced immunogenicity in tumor and circulation of high-risk melanoma patients with unknown primary.Journal for immunotherapy of cancer · 2022Trial
- Trial
- Circulating Cytokines in Melanoma Prognosis: Current Evidence and Future Perspectives.Medicina (Kaunas, Lithuania) · 2026Review
- TSIX: A Novel Long Noncoding RNA in Cancer Development and Progression.Current pharmaceutical design · 2026Review
- Effects of hesperidin supplementation on inflammation and oxidative stress in overweight or obese individuals: a systematic review and meta-analysis of randomized controlled trials.Frontiers in nutrition · 2026Review
- Cross-sectional evaluation of exposure to ozone, nitrogen dioxide, and particulate mass levels on circulating immune markers in women in the California Teachers Study.Scientific reports · 2025Article
- Cross-sectional evaluation of exposure to ozone, nitrogen dioxide, and particulate mass levels on circulating immune markers in women in the California Teachers Study.Research square · 2025Article
- Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing.Journal of immunological methods · 2024Article
- Evaluating the long-term biological stability of cytokine biomarkers in ocular fluid samples.BMJ open ophthalmology · 2023Article
- Multiple Cytokine Analysis of Th1/Th2/Th9/Th17/Th22/Treg Cytokine Pathway for Individual Immune Profile Assessment in Patients with Psoriasis.Medical science monitor : international medical journal of experimental and clinical research · 2022Article
- Enhanced immune activation within the tumor microenvironment and circulation of female high-risk melanoma patients and improved survival with adjuvant CTLA4 blockade compared to males.Journal of translational medicine · 2022Article
- Tumor Biomarker In-Solution Quantification, Standard Production, and Multiplex Detection.Journal of immunology research · 2021Review
- Evaluation of Host Serum Protein Biomarkers of Tuberculosis in sub-Saharan Africa.Frontiers in immunology · 2021Article
- Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage.Biology · 2020Article
- XIST and TSIX: Novel Cancer Immune Biomarkers in PD-L1-Overexpressing Breast Cancer Patients.Frontiers in oncology · 2019Article
- Effects of freezer storage time on levels of complement biomarkers.BMC research notes · 2017Article
- Stability of pro- and anti-inflammatory immune biomarkers for human cohort studies.Journal of translational medicine · 2017Article
- Psychiatric disorders biochemical pathways unraveled by human brain proteomics.European archives of psychiatry and clinical neuroscience · 2017Review
- Wound samples: moving towards a standardised method of collection and analysis.International wound journal · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
backgroundIdentification of predictive and prognostic biomarkers for patients with disease and undergoing different therapeutic options is a very active area of investigation. Many of these studies seek biomarkers among circulating proteins accessed in blood. Many levels of standardization in materials and procedures have been identified which can impact the resulting data.
methodsHere, we have observed unexpected variability in levels of commonly tested analytes in serum which were processed and stored under standardized conditions. We have identified apparent changes in cytokine, chemokine and growth factor levels detected by multiplex Luminex assay in melanoma patient and healthy donor serum samples, over storage time at -80°C. Controls included Luminex kit standards, multiplexed cytokine standards and WHO cytokine controls. Data were analyzed by Wilcoxon rank-sum testing and Spearman's test for correlations.
resultsThe interpretation of these changes is confounded by lot-to-lot kit standard curve reagent changes made by a single manufacturer of Luminex kits.
conclusionsThis study identifies previously unknown sources of variation in a commonly used biomarker assay, and suggests additional levels of controls needed for identification of true changes in circulating protein levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.