ArticleBMC genomics2011
Analysis of conserved microRNAs in floral tissues of sexual and apomictic Boechera species.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Integrative gene expression and heterologous functional analysis identify candidate regulators of apomixis inFrontiers in plant science · 2026Article
- New insights intoFrontiers in plant science · 2025Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- A combined transcriptome - miRNAome approach revealed that aFrontiers in plant science · 2022Article
- The emerging role of small RNAs in ovule development, a kind of magic.Plant reproduction · 2021Review
- A study of the heterochronic sense/antisense RNA representation in florets of sexual and apomictic Paspalum notatum.BMC genomics · 2021Article
- Article
- Review
- Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula.BMC genomics · 2019Article
- Small RNA-seq reveals novel regulatory components for apomixis in Paspalum notatum.BMC genomics · 2019Article
- TheFrontiers in plant science · 2019Review
- Diplosporous development in Boehmeria tricuspis: Insights from de novo transcriptome assembly and comprehensive expression profiling.Scientific reports · 2017Article
- Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation.BMC biology · 2016Article
- Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis.Journal of experimental botany · 2016Article
- Apomictic and sexual germline development differ with respect to cell cycle, transcriptional, hormonal and epigenetic regulation.PLoS genetics · 2014Article
- The genetic control of apomixis: asexual seed formation.Genetics · 2014Review
- Article
- The conserved chimeric transcript UPGRADE2 is associated with unreduced pollen formation and is exclusively found in apomictic Boechera species.Plant physiology · 2013Article
- Computational identification of conserved microRNAs and their putative targets in the Hypericum perforatum L. flower transcriptome.Plant reproduction · 2013Article
- High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot.BMC genomics · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApomixis or asexual seed formation represents a potentially important agronomic trait whose introduction into crop plants could be an effective way to fix and perpetuate a desirable genotype through successive seed generations. However, the gene regulatory pathways underlying apomixis remain unknown. In particular, the potential function of microRNAs, which are known to play crucial roles in many aspects of plant growth and development, remains to be determined with regards to the switch from sexual to apomictic reproduction.
resultsUsing bioinformatics and microarray validation procedures, 51 miRNA families conserved among angiosperms were identified in Boechera. Microarray assay confirmed 15 of the miRNA families that were identified by bioinformatics techniques. 30 cDNA sequences representing 26 miRNAs could fold back into stable pre-miRNAs. 19 of these pre-miRNAs had miRNAs with Boechera-specific nucleotide substitutions (NSs). Analysis of the Gibbs free energy (ΔG) of these pre-miRNA stem-loops with NSs showed that the Boechera-specific miRNA NSs significantly (p ≤ 0.05) enhance the stability of stem-loops. Furthermore, six transcription factors, the Squamosa promoter binding protein like SPL6, SPL11 and SPL15, Myb domain protein 120 (MYB120), RELATED TO AP2.7 DNA binding (RAP2.7, TOE1 RAP2.7) and TCP family transcription factor 10 (TCP10) were found to be expressed in sexual or apomictic ovules. However, only SPL11 showed differential expression with significant (p ≤ 0.05) up-regulation at the megaspore mother cell (MMC) stage of ovule development in apomictic genotypes.
conclusionsThis study constitutes the first extensive insight into the conservation and expression of microRNAs in Boechera sexual and apomictic species. The miR156/157 target squamosa promoter binding protein-like 11 (SPL11) was found differentially expressed with significant (p ≤ 0.05) up-regulation at the MMC stage of ovule development in apomictic genotypes. The results also demonstrate that nucleotide changes in mature miRNAs significantly (p ≤ 0.05) enhance the thermodynamic stability of pre-miRNA stem-loops.
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