ArticlePlant physiology2011
The transcription factor CrWRKY1 positively regulates the terpenoid indole alkaloid biosynthesis in Catharanthus roseus.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 190 papers.
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190 citing papers in PubMed, 428 citations in OpenAlex.
- Medicinal-plant immunity and specialized metabolism in plant-pathogen interactions: mechanisms and applications.Planta · 2026Review
- Transcriptional Regulation of the Novel Theacrine Synthase Gene CsTcS2 by the CsTINY-CsWRKY33 Module Underpins Theacrine Biosynthesis in Camellia sinensis.Plant biotechnology journal · 2026Article
- Metabolic engineering of tobacco plants using Carica papaya NAC transcription factors: a biotechnology tool to improve plant metabolite production.Biotechnology letters · 2026Article
- Co-Overexpression ofPlants (Basel, Switzerland) · 2026Article
- Positive regulatory role of EuWRKY1 in the biosynthesis of Eucommia ulmoides rubber.Functional & integrative genomics · 2026Article
- mRNA sequencing revealed transcriptional variability of terpene and eugenol synthase genes among distinct oil yielding accessions of Ocimum sanctum L.Scientific reports · 2026Article
- Genome-Wide Identification of WRKY Gene Family in Artemisia and Its Expression Analysis of Aphid Resistance.International journal of molecular sciences · 2026Article
- The genome of the medicinal plant Uncaria rhynchophylla provides new insights into monoterpenoid indole alkaloid metabolism and its molecular regulatory mechanism.Molecular horticulture · 2026Article
- Combined Transcriptomic and Metabolomic Analysis Reveals an Ethylene-Activated Regulatory Model on Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.Plant biotechnology journal · 2026Article
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- Mining the candidate transcription factors modulating dendrobine biosynthesis under phosphate deficiency inFrontiers in plant science · 2026Article
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- Genome-wide characterization of WRKYs and identification of SpWRKY40 and SpWRKY51 as transcription activators in Sesuvium portulacastrum.Functional & integrative genomics · 2025Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- An HDG11-mediated transcriptional network regulates 1-deoxynojirimycin biosynthesis in mulberry.Plant physiology · 2025Article
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- Salicylic acid regulates biosynthesis of floral fragrance (E)-β-farnesene via NPR3-WRKY1 module in chrysanthemum.Molecular horticulture · 2025Article
- The PlMYB73-PlMYB70-PlMYB108 complex regulatesHorticulture research · 2025Article
- Hepatocytes as Model for Investigating Natural Senotherapeutic Compounds and Their Effects on Cell Cycle Dynamics and Genome Stability.International journal of molecular sciences · 2025Review
- Biosynthetic Machinery to Abiotic Stress-Driven Emission: Decoding Multilayer Regulation of Volatile Terpenoids in Plants.Antioxidants (Basel, Switzerland) · 2025Review
130 more citing papers are in PubMed but not listed here.
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6 authors at 2 institutions in 1 country.
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Abstract
Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source of natural or semisynthetic anticancer drugs. The biosynthesis of TIAs is tissue specific and induced by certain phytohormones and fungal elicitors, indicating the involvement of a complex transcriptional control network. However, the transcriptional regulation of the TIA pathway is poorly understood. Here, we describe a C. roseus WRKY transcription factor, CrWRKY1, that is preferentially expressed in roots and induced by the phytohormones jasmonate, gibberellic acid, and ethylene. The overexpression of CrWRKY1 in C. roseus hairy roots up-regulated several key TIA pathway genes, especially Tryptophan Decarboxylase (TDC), as well as the transcriptional repressors ZCT1 (for zinc-finger C. roseus transcription factor 1), ZCT2, and ZCT3. However, CrWRKY1 overexpression repressed the transcriptional activators ORCA2, ORCA3, and CrMYC2. Overexpression of a dominant-repressive form of CrWRKY1, created by fusing the SRDX repressor domain to CrWRKY1, resulted in the down-regulation of TDC and ZCTs but the up-regulation of ORCA3 and CrMYC2. CrWRKY1 bound to the W box elements of the TDC promoter in electrophoretic mobility shift, yeast one-hybrid, and C. roseus protoplast assays. Up-regulation of TDC increased TDC activity, tryptamine concentration, and resistance to 4-methyl tryptophan inhibition of CrWRKY1 hairy roots. Compared with control roots, CrWRKY1 hairy roots accumulated up to 3-fold higher levels of serpentine. The preferential expression of CrWRKY1 in roots and its interaction with transcription factors including ORCA3, CrMYC2, and ZCTs may play a key role in determining the root-specific accumulation of serpentine in C. roseus plants.
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