ArticleProceedings of the National Academy of Sciences of the United States of America2011
Subunit stoichiometry of human Orai1 and Orai3 channels in closed and open states.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 88 citations in OpenAlex.
- Calcium Signaling Dysregulation in Diabetic Cardiomyopathy: Roles of STIM and Orai Channels.Handbook of experimental pharmacology · 2026Review
- MaxCal can infer models from coupled stochastic trajectories of gene expression and cell division.Biophysical journal · 2023Article
- Pore-Forming Proteins: From Pore Assembly to Structure by Quantitative Single-Molecule Imaging.International journal of molecular sciences · 2023Review
- Similarities and Differences between the Orai1 Variants: Orai1α and Orai1β.International journal of molecular sciences · 2022Review
- Modeling Non-additive Effects in Neighboring Chemically Identical Fluorophores.The journal of physical chemistry. B · 2022Article
- The interplay between mitochondria and store-operated CaJournal of cellular and molecular medicine · 2021Review
- The store-operated CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation.International journal of molecular sciences · 2021Article
- Store-Operated Calcium Channels: From Function to Structure and Back Again.Cold Spring Harbor perspectives in biology · 2020Review
- The Number and Position of Orai3 Units within Heteromeric Store-Operated CaInternational journal of molecular sciences · 2020Article
- Numbers count: How STIM and Orai stoichiometry affect store-operated calcium entry.Cell calcium · 2019Review
- Differentiation-Induced Remodelling of Store-Operated Calcium Entry Is Independent of Neuronal or Glial Phenotype but Modulated by Cellular Context.Molecular neurobiology · 2019Article
- Maximum Caliber Can Build and Infer Models of Oscillation in a Three-Gene Feedback Network.The journal of physical chemistry. B · 2019Article
- STIM1 activation of Orai1.Cell calcium · 2019Review
- Pore properties of Orai1 calcium channel dimers and their activation by the STIM1 ER calcium sensor.The Journal of biological chemistry · 2018Article
- CALHM3 Is Essential for Rapid Ion Channel-Mediated Purinergic Neurotransmission of GPCR-Mediated Tastes.Neuron · 2018Article
- Stanniocalcin 2 Regulates Non-capacitative CaFrontiers in physiology · 2018Article
- Building Predictive Models of Genetic Circuits Using the Principle of Maximum Caliber.Biophysical journal · 2017Article
- A quantized mechanism for activation of pannexin channels.Nature communications · 2017Article
- Orai1, 2, 3 and STIM1 promote store-operated calcium entry in pulmonary arterial smooth muscle cells.Cell death discovery · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
We applied single-molecule photobleaching to investigate the stoichiometry of human Orai1 and Orai3 channels tagged with eGFP and expressed in mammalian cells. Orai1 was detected predominantly as dimers under resting conditions and as tetramers when coexpressed with C-STIM1 to activate Ca(2+) influx. Orai1 was also found to be tetrameric when coexpressed with STIM1 and evaluated following fixation. We show that fixation rapidly causes release of Ca(2+), redistribution of STIM1 to the plasma membrane, and STIM1/Orai1 puncta formation, and may cause the channel to be in the activated state. Consistent with this possibility, Orai1 was found predominantly as a dimer when coexpressed with STIM1 in living cells under resting conditions. We further show that Orai3, like Orai1, is dimeric under resting conditions and is predominantly tetrameric when activated by C-STIM1. Interestingly, a dimeric Orai3 stoichiometry was found both before and during application of 2-aminoethyldiphenyl borate (2-APB) to activate a nonselective cation conductance in its STIM1-independent mode. We conclude that the human Orai1 and Orai3 channels undergo a dimer-to-tetramer transition to form a Ca(2+)-selective pore during store-operated activation and that Orai3 forms a dimeric nonselective cation pore upon activation by 2-APB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.