ArticleProceedings of the National Academy of Sciences of the United States of America2011
Carbon and nitrogen assimilation in deep subseafloor microbial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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87 citing papers in PubMed.
- Heterotrophic inorganic carbon fixation varies with temperature and resource availability in marine and soil bacterial isolates.Applied and environmental microbiology · 2026Article
- Oxic Subseafloor Microbial Communities Retain Anabolic Capacity under both Oxic and Oxygen-depleted Conditions.Microbes and environments · 2026Article
- Subsurface Life on Earth as a Key to Unlock Extraterrestrial Mysteries.Microbial biotechnology · 2025Review
- Moderate heating renders 7.8-million-year-old sedimentary organic matter bioavailable.Science advances · 2025Article
- Article
- A global comparison of surface and subsurface microbiomes reveals large-scale biodiversity gradients, and a marine-terrestrial divide.Science advances · 2024Article
- Single-cell measurement of microbial growth rate with Raman microspectroscopy.FEMS microbiology ecology · 2024Article
- Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin.FEMS microbiology ecology · 2024Article
- Single-cell analysis reveals an active and heterotrophic microbiome in the Guaymas Basin deep subsurface with significant inorganic carbon fixation by heterotrophs.Applied and environmental microbiology · 2024Article
- Microbial eukaryotic predation pressure and biomass at deep-sea hydrothermal vents.The ISME journal · 2024Article
- Genomic insights into Penicillium chrysogenum adaptation to subseafloor sedimentary environments.BMC genomics · 2024Article
- The subseafloor crustal biosphere: Ocean's hidden biogeochemical reactor.Frontiers in microbiology · 2024Review
- Assessment of prokaryotic communities in Southwestern Atlantic deep-sea sediments reveals prevalent methanol-oxidising Methylomirabilales.Scientific reports · 2023Article
- From Minerals to Simplest Living Matter: Life Origination Hydrate Theory.Acta biotheoretica · 2023Article
- Deep Subseafloor Biogeochemical Processes and Microbial Populations Potentially Associated with the 2011 Tohoku-oki Earthquake at the Japan Trench Accretionary Wedge (IODP Expedition 343).Microbes and environments · 2023Article
- Variations in Marine Bacterial and Archaeal Communities during anMicroorganisms · 2022Article
- Metabolic cross-feeding structures the assembly of polysaccharide degrading communities.Science advances · 2022Article
- Water is a preservative of microbes.Microbial biotechnology · 2022Article
- Cryptic metabolisms in anoxic subseafloor sediment.Environmental microbiology reports · 2021Article
- Genomes of Thaumarchaeota from deep sea sediments reveal specific adaptations of three independently evolved lineages.The ISME journal · 2021Article
27 more citing papers are in PubMed but not listed here.
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Remarkable numbers of microbial cells have been observed in global shallow to deep subseafloor sediments. Accumulating evidence indicates that deep and ancient sediments harbor living microbial life, where the flux of nutrients and energy are extremely low. However, their physiology and energy requirements remain largely unknown. We used stable isotope tracer incubation and nanometer-scale secondary ion MS to investigate the dynamics of carbon and nitrogen assimilation activities in individual microbial cells from 219-m-deep lower Pleistocene (460,000 y old) sediments from the northwestern Pacific off the Shimokita Peninsula of Japan. Sediment samples were incubated in vitro with (13)C- and/or (15)N-labeled glucose, pyruvate, acetate, bicarbonate, methane, ammonium, and amino acids. Significant incorporation of (13)C and/or (15)N and growth occurred in response to glucose, pyruvate, and amino acids (∼76% of total cells), whereas acetate and bicarbonate were incorporated without fostering growth. Among those substrates, a maximum substrate assimilation rate was observed at 67 × 10(-18) mol/cell per d with bicarbonate. Neither carbon assimilation nor growth was evident in response to methane. The atomic ratios between nitrogen incorporated from ammonium and the total cellular nitrogen consistently exceeded the ratios of carbon, suggesting that subseafloor microbes preferentially require nitrogen assimilation for the recovery in vitro. Our results showed that the most deeply buried subseafloor sedimentary microbes maintain potentials for metabolic activities and that growth is generally limited by energy but not by the availability of C and N compounds.
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