ArticleThe Journal of infectious diseases2011
Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread.
Article in The Journal of infectious diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- A newly developed eGFP-traceable recombinant rhesus lymphocryptovirus, a tool to study viral infection and replicationJournal of virology · 2026Article
- Recovery of Recombinant Marburg Virus by Reverse Genetics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Labeling Ebola Virus with a Self-Splicing Fluorescent Reporter.Microorganisms · 2022Article
- Assessment of Life Cycle Modeling Systems as Prediction Tools for a Possible Attenuation of Recombinant Ebola Viruses.Viruses · 2022Article
- A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses.Cell reports · 2018Article
- Marburg Virus Reverse Genetics Systems.Viruses · 2016Review
- Reverse genetics systems as tools for the development of novel therapies against filoviruses.Expert review of anti-infective therapy · 2014Review
- Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA.Archives of virology · 2014Article
- Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.Virology · 2013Article
- A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit.Veterinary research · 2013Article
- Live-cell imaging of Marburg virus-infected cells uncovers actin-dependent transport of nucleocapsids over long distances.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Ebola virus does not block apoptotic signaling pathways.Journal of virology · 2013Article
- Forty-five years of Marburg virus research.Viruses · 2012Review
- Review
- Filovirus Strategies to Escape Antiviral Responses.Current topics in microbiology and immunologyReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The generation of recombinant enhanced green fluorescent protein (EGFP)-expressing viruses has significantly improved the study of their life cycle and opened up the possibility for the rapid screening of antiviral drugs. Here we report rescue of a recombinant Marburg virus (MARV) expressing EGFP from an additional transcription unit (ATU). The ATU was inserted between the second and third genes, encoding VP35 and VP40, respectively. Live-cell imaging was used to follow virus spread in real time. EGFP expression was detected at 32 hours postinfection (hpi), and infection of neighboring cells was monitored at 55 hpi. Compared to the parental virus, production of progeny rMARV-EGFP was reduced 4-fold and lower protein levels of VP40, but not nucleoprotein, were observed, indicating a decrease in downstream protein expression due to the insertion of an ATU. Interestingly, EGFP concentrated in viral inclusions in infected cells. This was reproduced by transient expression of both EGFP and other fluorescent proteins along with filovirus nucleocapsid proteins, and may suggest that a general increase in protein synthesis occurs at viral inclusion sites. In conclusion, the EGFP-expressing MARV will be a useful tool not only to monitor virus spread and screen for antiviral compounds, but also to investigate the biology of inclusion body formation.
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