ArticleThe Journal of biological chemistry2011
Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.Journal of hematology & oncology · 2021Review
- LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.Science advances · 2019Article
- Downregulation of HDGF inhibits the tumorigenesis of bladder cancer cells by inactivating the PI3K-AKT signaling pathway.Cancer management and research · 2019Article
- Interactions between Retroviruses and the Host Cell Genome.Molecular therapy. Methods & clinical development · 2018Review
- Nuclear landscape of HIV-1 infection and integration.Nature reviews. Microbiology · 2017Review
- Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry.Scientific reports · 2016Article
- The significance of autoantibodies to DFS70/LEDGFp75 in health and disease: integrating basic science with clinical understanding.Clinical and experimental medicine · 2016Review
- Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.Nucleic acids research · 2016Article
- Towards a Safer, More Randomized Lentiviral Vector Integration Profile Exploring Artificial LEDGF Chimeras.PloS one · 2016Article
- Article
- LEDGF/p75 interacts with mRNA splicing factors and targets HIV-1 integration to highly spliced genes.Genes & development · 2015Article
- Impact of Chromatin on HIV Replication.Genes · 2015Review
- Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif.Nature communications · 2015Article
- Human immunodeficiency virus (HIV)-1 integration sites in viral latency.Current HIV/AIDS reports · 2015Review
- HIV-1 IN/Pol recruits LEDGF/p75 into viral particles.Retrovirology · 2015Article
- The expression and clinical significance of HDGF in osteosarcoma.OncoTargets and therapy · 2015Article
- Interrogating HIV integrase for compounds that bind--a SAMPL challenge.Journal of computer-aided molecular design · 2014Article
- Molecular mechanisms of retroviral integration site selection.Nucleic acids research · 2014Article
- Repair of oxidative DNA base damage in the host genome influences the HIV integration site sequence preference.PloS one · 2014Article
- Article
Corrections and comments
- Retraction · 2017-12-13Duplication of/in Image ·
- Retracted
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
LEDGF/p75 is a chromatin-interacting, cellular cofactor of HIV integrase that dictates lentiviral integration site preference. In this study we determined the role of the PWWP domain of LEDGF/p75 in tethering and targeting of the lentiviral pre-integration complex, employing potent knockdown cell lines allowing analysis in the absence of endogenous LEDGF/p75. Deletion of the PWWP domain resulted in a diffuse subnuclear distribution pattern, loss of interaction with condensed chromatin, and failure to rescue proviral integration, integration site distribution, and productive virus replication. Substitution of the PWWP domain of LEDGF/p75 with that of hepatoma-derived growth factor or HDGF-related protein-2 rescued viral replication and lentiviral integration site distribution in LEDGF/p75-depleted cells. Replacing all chromatin binding elements of LEDGF/p75 with full-length hepatoma-derived growth factor resulted in more integration in genes combined with a preference for CpG islands. In addition, we showed that any PWWP domain targets SMYD1-like sequences. Analysis of integration preferences of lentiviral vectors for epigenetic marks indicates that the PWWP domain is critical for interactions specifying the relationship of integration sites to regions enriched in specific histone post-translational modifications.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.