ArticleMolecular biotechnology2012
Differentiating ovine BSE from CH1641 scrapie by serial protein misfolding cyclic amplification.
Article in Molecular biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Classical BSE dismissed as the cause of CWD in Norwegian red deer despite strain similarities between both prion agents.Veterinary research · 2024Article
- Site-specific analysis of N-glycans from different sheep prion strains.PLoS pathogens · 2021Article
- Genetic resistance to transmissible spongiform encephalopathies (TSE) in goats.EFSA journal. European Food Safety Authority · 2017Article
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- Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification.Journal of clinical microbiology · 2014Article
- In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation.BMC veterinary research · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V₁₃₆R₁₅₄Q₁₇₁/V₁₃₆R₁₅₄Q₁₇₁ or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.
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Registered trials
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