ArticlePediatric surgery international2012
Oral insulin stimulates intestinal epithelial cell turnover following massive small bowel resection in a rat and a cell culture model.
Article in Pediatric surgery international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- An Overview of Short-Bowel Syndrome in Pediatric Patients: Focus on Clinical Management and Prevention of Complications.Nutrients · 2023Review
- The Imaging Features and Diagnostic Value of Computerised Tomography in Seminal Duct Tuberculosis.Journal of multidisciplinary healthcare · 2023Article
- Nutritional and pharmacological strategy in children with short bowel syndrome.Pediatric surgery international · 2021Review
- Short Bowel Syndrome as the Leading Cause of Intestinal Failure in Early Life: Some Insights into the Management.Pediatric gastroenterology, hepatology & nutrition · 2019Review
- Bile salt dependent lipase promotes intestinal adaptation in rats with massive small bowel resection.Bioscience reports · 2018Article
- Epithelial IGF1R is dispensable for IGF2 mediated enhanced intestinal adaptation in retinoblastoma-deficient mice.Journal of pediatric surgery · 2017Article
- A Novel Role of Spred2 in the Colonic Epithelial Cell Homeostasis and Inflammation.Scientific reports · 2016Article
- MEK/ERK pathway activation by insulin receptor isoform alteration is associated with the abnormal proliferation and differentiation of intestinal epithelial cells in diabetic mice.Molecular and cellular biochemistry · 2016Article
- Deletion of intestinal epithelial insulin receptor attenuates high-fat diet-induced elevations in cholesterol and stem, enteroendocrine, and Paneth cell mRNAs.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Insulin-like growth factor 2 and its enterocyte receptor are not required for adaptation in response to massive small bowel resection.Journal of pediatric surgery · 2014Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeWe have recently reported that oral insulin (OI) stimulates intestinal adaptation after bowel resection and that OI enhances enterocyte turnover in correlation with insulin receptor expression along the villus-crypt axis. The purpose of the present study was to evaluate the effect of OI on intestinal epithelial cell proliferation and apoptosis in a rat model of short bowel syndrome (SBS) and in a cell culture model.
methodsCaco-2 cells were incubated with increasing concentrations of insulin. Cell proliferation and apoptosis were determined by FACS cytometry. Cell viability was investigated using the Alamar Blue technique. Male rats were divided into three groups: Sham rats underwent bowel transection, SBS rats underwent a 75% bowel resection, and SBS-OI rats underwent bowel resection and were treated with OI given in drinking water (1 U/ml) from the third postoperative day. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined on day 15. Real time PCR was used to determine the level of bax and bcl-2 mRNA and western blotting was used to determine bax, bcl-2, p-ERK and AKT protein levels. Statistical analysis was performed using the one-way ANOVA test, with P < 0.05 considered statistically significant.
resultsTreatment of Caco-2 cells with insulin resulted in a significant increase in cell proliferation (twofold increase after 24 h and 37% increase after 48 h) and cell viability (in a dose-dependent manner), but did not change cell apoptosis. In a rat model of SBS, treatment with OI resulted in a significant increase in all parameters of intestinal adaptation. Elevated cell proliferation rate in insulin treated rats was accompanied by elevated AKT and p-ERK protein levels. Decreased cell apoptosis in SBS-INS rats corresponded with a decreased bax/bcl-2 ratio.
conclusionsOral insulin stimulates intestinal epithelial cell turnover after massive small bowel resection in a rat model of SBS and a cell culture model.
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