ArticleJournal of immunological methods2012
Quantitative analysis of T cell receptor diversity in clinical samples of human peripheral blood.
Article in Journal of immunological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Signatures of T cell immunity revealed using sequence similarity with TCRDivER algorithm.Communications biology · 2023Article
- Contribution of the TCRβ Repertoire to Marek's Disease Genetic Resistance in the Chicken.Viruses · 2023Article
- Recent Advancements in Regenerative Approaches for Thymus Rejuvenation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Validation of a Next-Generation Sequencing-Based T-Cell Receptor Gamma Gene Rearrangement Diagnostic Assay: Transitioning from Capillary Electrophoresis to Next-Generation Sequencing.The Journal of molecular diagnostics : JMD · 2021Article
- Partial recovery of disturbed V-J pairing profiles of T-cell receptor in people living with HIV receiving long-term antiretroviral therapy.Science China. Life sciences · 2021Article
- The era of immunogenomics/immunopharmacogenomics.Journal of human genetics · 2018Review
- Analysis of T cell receptor repertoire based on Vβ chain in patients with breast cancer.Cancer biomarkers : section A of Disease markers · 2018Article
- Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq.Genome medicine · 2016Article
- Extracoporeal photopheresis treatment of acute graft-versus-host disease following allogeneic haematopoietic stem cell transplantation.F1000Research · 2016Review
- Donor CD4 T Cell Diversity Determines Virus Reactivation in Patients After HLA-Matched Allogeneic Stem Cell Transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2015Article
- Methods for diversity and overlap analysis in T-cell receptor populations.Journal of mathematical biology · 2013Article
- Flow cytometric analysis of the CD4+ TCR Vβ repertoire in the peripheral blood of children with type 1 diabetes mellitus, systemic lupus erythematosus and age-matched healthy controls.BMC immunology · 2013Article
- Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation.Nature medicine · 2013Article
- Monitoring the Dynamics of T Cell Clonal Diversity Using Recombinant Peptide:MHC Technology.Frontiers in immunology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The analysis of T cell receptor diversity provides a clinically relevant and sensitive marker of repertoire loss, gain, or skewing. Spectratyping is a broadly utilized technique to measure global TCR diversity by the analysis of the lengths of CDR3 fragments in each Vβ family. However the common use of large numbers of T cells to obtain a global view of TCR Vβ CDR3 diversity has restricted spectratyping analyses when limited T-cell numbers are available in clinical setting, such as following transplant regimens. We here demonstrate that one hundred thousand T cells are sufficient to obtain a robust, highly reproducible measure of the global TCR Vβ repertoire diversity among twenty Vβ families in human peripheral blood. We also show that use of lower cell number results not in a dwindling of observed diversity but rather in non-reproducible patterns in replicate spectratypes. Finally, we report here a simple to use but sensitive method to quantify repertoire divergence in patient samples by comparison to a standard repertoire profile we generated from fifteen normal donors. We provide examples using this method to statistically evaluate the changes in the global TCR Vβ repertoire diversity that may take place during T subset immune reconstitution after hematopoietic stem cell transplantation or after immune modulating therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.