ArticleThe Indian journal of medical research2011
γδ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence.
Article in The Indian journal of medical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics.NPJ vaccines · 2024Article
- Evidence of innate training in bovine γδ T cells following subcutaneous BCG administration.Frontiers in immunology · 2024Article
- Expression, Purification, and Crystallization of the Vγ9Vδ2 T-cell Receptor Recognizing Protein/Peptide Antigens.The protein journal · 2023Article
- Defining TCRγδ lymphoproliferative disorders by combined immunophenotypic and molecular evaluation.Nature communications · 2022Article
- Non-structure protein ORF1ab (NSP8) in SARS-CoV-2 contains potential γδT cell epitopes.Frontiers in microbiology · 2022Article
- It Takes a Village: The Multifaceted Immune Response toFrontiers in immunology · 2022Review
- Review
- Identification of new potential antigen recognized by γδT cells in hepatocellular carcinoma.Cancer immunology, immunotherapy : CII · 2021Article
- A Cell for the Ages: Human γδ T Cells across the Lifespan.International journal of molecular sciences · 2020Review
- Profiling the pattern of the human T-cell receptor γδ complementary determinant region 3 repertoire in patients with lung carcinoma via high-throughput sequencing analysis.Cellular & molecular immunology · 2019Article
- Next generation sequencing reveals changes of the γδ T cell receptor repertoires in patients with pulmonary tuberculosis.Scientific reports · 2018Article
- Development and Selection of the Human Vγ9Vδ2Frontiers in immunology · 2018Review
- Next-Generation Sequencing Analysis of the Human TCRγδ+ T-Cell Repertoire Reveals Shifts in Vγ- and Vδ-Usage in Memory Populations upon Aging.Frontiers in immunology · 2018Article
- Characteristics of the Vδ2 CDR3 Sequence of Peripheral γδ T Cells in Patients with Pulmonary Tuberculosis and Identification of a New Tuberculosis-Related Antigen Peptide.Clinical and vaccine immunology : CVI · 2015Article
- Identification of a new tuberculosis antigen recognized by γδ T cell receptor.Clinical and vaccine immunology : CVI · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesThe unique immunological functions of γδ T lymphocytes to contribute immunity against Mycobacterium tuberculosis attracted interest of researchers. However, little is known about the specificity of γδ Τ cell in tuberculosis patients and the lack of exact tuberculosis antigen recognized by γδ T cells limited its application. The analysis of complementary determinant region (CDR)3 sequence characteristic in γδ T cells of tuberculosis patients would contribute to understand the distribution specificity of γδ T cell. In present study, we investigated the diversity of the γ9/δ2 T cell immunorepertoire and analysed the specificity of the expressed CDR3 in pulmonary tuberculosis patients.
methodsThe total RNA in peripheral blood mononuclear cell of 50 pulmonary tuberculosis patients and 10 healthy controls was extracted. The polymerase chain reaction was used to specifically amplify the CDR3 region of γ9 and δ2 chain. The PCR products were ligated into the pGEM-T easy vector. The plasmid DNA was sequenced using the ABI3700 and the T7 primer.
resultsOur findings showed that predominant CDR3 sequence of δ2 chain in pulmonary tuberculosis patients was CACDTLVSTDKLIFGKG. The sequence specifically exists in almost all pulmonary tuberculosis patients. The conserved hydrophobic acid residue in 97 positions is present in the γδ T cell reactive to M. tuberculosis. The length of δ2 CDR3 in pulmonary tuberculosis patients has no relation with the disease progress. INTERPRETATION &
conclusionsOur results suggest that γδ T cells appear to use CDR3 sequence to recognise M. tuberculosis antigen. γδ T cells reactive to M. tuberculosis were diverse and polyclonal.
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Identifiers
21985819PMC3193717W2146321191What OpenQuestion holds
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