ArticleJournal of neuroscience methods2012
Measuring CaMKII concentration in dendritic spines.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- CaMKII generates actin bundle morphology and mechanics distinct from canonical bivalent cross-linkers.bioRxiv : the preprint server for biology · 2026Article
- Article
- Ligand-induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity.Protein science : a publication of the Protein Society · 2024Article
- PKA regulation of neuronal function requires the dissociation of catalytic subunits from regulatory subunits.bioRxiv : the preprint server for biology · 2024Article
- Controlling the interaction between CaMKII and Calmodulin with a photocrosslinking unnatural amino acid.Protein science : a publication of the Protein Society · 2023Article
- Article
- Comparison of rule- and ordinary differential equation-based dynamic model of DARPP-32 signalling network.PeerJ · 2022Article
- Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Photoactivatable CaMKII induces synaptic plasticity in single synapses.Nature communications · 2021Article
- Characterization of CaMKIIα holoenzyme stability.Protein science : a publication of the Protein Society · 2020Article
- Architectural Dynamics of CaMKII-Actin Networks.Biophysical journal · 2019Article
- Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors.Science (New York, N.Y.) · 2018Article
- Criteria for identifying the molecular basis of the engram (CaMKII, PKMzeta).Molecular brain · 2017Review
- Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates.Cell reports · 2017Article
- Dysregulation of neuronal calcium homeostasis in Alzheimer's disease - A therapeutic opportunity?Biochemical and biophysical research communications · 2017Review
- A fluorescence assay for detecting amyloid-β using the cytomegalovirus enhancer/promoter.Journal of biological methods · 2017Article
- Multiple CaMKII Binding Modes to the Actin Cytoskeleton Revealed by Single-Molecule Imaging.Biophysical journal · 2016Article
- A novel explanation for observed CaMKII dynamics in dendritic spines with added EGTA or BAPTA.Biophysical journal · 2015Article
- Development of a molecularly evolved, highly sensitive CaMKII FRET sensor with improved expression pattern.PloS one · 2015Article
- Excitotoxic insult results in a long-lasting activation of CaMKIIα and mitochondrial damage in living hippocampal neurons.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Here, we present a method for measuring the concentration of endogenous protein in cellular compartments. Importantly, the method is applicable to compartments such as dendritic spines with dimensions often close to the resolution limit of optical microscopy. To our knowledge, a method with such capabilities has not yet been described. The method utilizes overexpression of the protein of interest, which is tagged with fluorescent protein. This is followed by immunostaining of both overexpressed and endogenous proteins. Expression of a volume marker is also required. We applied this method to measure the concentration of Ca/calmodulin kinase II (CaMKII) in different cellular compartments of hippocampal pyramidal neurons. It was found that the concentrations of CaMKIIα subunits in cell bodies, proximal dendrites, and spines on these dendrites are 71, 46, and 103 μM, respectively. Considering the 3:1 ratio of α to β CaMKII subunits in the hippocampus, the concentrations of total (α+β) CaMKII subunits in these compartments are 94, 61, and 138 μM, respectively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.