ArticlePloS one2011
Population structure of Pseudomonas aeruginosa from five Mediterranean countries: evidence for frequent recombination and epidemic occurrence of CC235.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Risk factors for colonisation and infection with multidrug-resistant Pseudomonas aeruginosa in intensive care unit: a systematic review and meta-analysis.Systematic reviews · 2026Pooled it
- Molecular characterization of serotype and virulence genes ofJournal of medical microbiology · 2025Article
- High-riskHeliyon · 2025Article
- Genomic insights into five selected multidrug-resistantFrontiers in microbiology · 2025Article
- Genomic and phenotypic characterisation ofFrontiers in microbiology · 2025Article
- Genomic virulence markers are associated with severe outcomes in patients with Pseudomonas aeruginosa bloodstream infection.Communications medicine · 2024Article
- Impact of multidrug resistance on the virulence and fitness of Pseudomonas aeruginosa: a microbiological and clinical perspective.Infection · 2024Review
- Population-based genomic surveillance of Pseudomonas aeruginosa causing bloodstream infections in a large Canadian health region.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Review
- Single nucleotide variants inMicrobial genomics · 2023Article
- Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis.Microbiology spectrum · 2023Article
- Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producingFrontiers in cellular and infection microbiology · 2023Article
- Antimicrobial resistance and genotyping ofFrontiers in veterinary science · 2023Article
- Risk factors for colonization and infection with multidrug-resistant Pseudomonas aeruginosa in intensive care unit: protocol for a systematic review and meta-analysis.Systematic reviews · 2022Article
- New Variants of PseudomonasMicroorganisms · 2022Article
- Whole-genome sequencing and gene sharing network analysis powered by machine learning identifies antibiotic resistance sharing between animals, humans and environment in livestock farming.PLoS computational biology · 2022Article
- Antibiotic Resistance in Pseudomonas.Advances in experimental medicine and biology · 2022Article
- Article
- A Potential High-Risk Clone ofFrontiers in microbiology · 2021Article
- Efficiency of a Tetracycline-Adjuvant Combination Against Multidrug ResistantAntibiotics (Basel, Switzerland) · 2020Article
8 more citing papers are in PubMed but not listed here.
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12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several studies in recent years have provided evidence that Pseudomonas aeruginosa has a non-clonal population structure punctuated by highly successful epidemic clones or clonal complexes. The role of recombination in the diversification of P. aeruginosa clones has been suggested, but not yet demonstrated using multi-locus sequence typing (MLST). Isolates of P. aeruginosa from five Mediterranean countries (n = 141) were subjected to pulsed-field gel electrophoresis (PFGE), serotyping and PCR targeting the virulence genes exoS and exoU. The occurrence of multi-resistance (≥ 3 antipseudomonal drugs) was analyzed with disk diffusion according to EUCAST. MLST was performed on a subset of strains (n = 110) most of them had a distinct PFGE variant. MLST data were analyzed with Bionumerics 6.0, using minimal spanning tree (MST) as well as eBURST. Measurement of clonality was assessed by the standardized index of association (I(A) (S)). Evidence of recombination was estimated by ClonalFrame as well as SplitsTree4.0. The MST analysis connected 70 sequence types, among which ST235 was by far the most common. ST235 was very frequently associated with the O11 serotype, and frequently displayed multi-resistance and the virulence genotype exoS⁻/exoU⁺. ClonalFrame linked several groups previously identified by eBURST and MST, and provided insight to the evolutionary events occurring in the population; the recombination/mutation ratio was found to be 8.4. A Neighbor-Net analysis based on the concatenated sequences revealed a complex network, providing evidence of frequent recombination. The index of association when all the strains were considered indicated a freely recombining population. P. aeruginosa isolates from the Mediterranean countries display an epidemic population structure, particularly dominated by ST235-O11, which has earlier also been coupled to the spread of ß-lactamases in many countries.
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