ArticleJournal of bacteriology2011
Deletion of the aconitase gene in Corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- DtxR is a global iron-dependent regulatory protein with multiple roles in the control of gene expression inJournal of bacteriology · 2026Article
- Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms.Biotechnology for biofuels and bioproducts · 2026Article
- Metabolic engineering of Corynebacterium glutamicum for acetate-based itaconic acid production.Biotechnology for biofuels and bioproducts · 2022Article
- Article
- High-Quality Genome-Scale Reconstruction ofFrontiers in microbiology · 2021Article
- Itaconic acid production in microorganisms.Biotechnology letters · 2018Review
- A new genome-scale metabolic model ofBiotechnology for biofuels · 2017Article
- Click-chemistry approach to study mycoloylated proteins: Evidence for PorB and PorC porins mycoloylation in Corynebacterium glutamicum.PloS one · 2017Article
- Secreted Citrate Serves as Iron Carrier for the Marine PathogenFrontiers in cellular and infection microbiology · 2017Article
- Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range.Microbial cell factories · 2015Article
- The ins and outs of bacterial iron metabolism.Molecular microbiology · 2014Review
- Oxidative metabolism enables Salmonella evasion of the NLRP3 inflammasome.The Journal of experimental medicine · 2014Article
- Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology.Applied and environmental microbiology · 2013Article
- Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.The Journal of biological chemistry · 2013Article
- Two roles for aconitase in the regulation of tricarboxylic acid branch gene expression in Bacillus subtilis.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aconitase gene acn of Corynebacterium glutamicum is regulated by four transcriptional regulators, indicating that the synthesis of this enzyme is carefully controlled. To understand the causes for this elaborate regulation, the properties of the Δacn-1 deletion mutant were analyzed in detail. The mutant was glutamate auxotrophic in glucose minimal medium, showed a strong growth defect, and secreted large amounts of acetate. None of these phenotypes could be complemented by plasmid-encoded aconitase, suggesting the presence of a secondary mutation. In fact, a point mutation within the gltA gene encoding citrate synthase was identified that caused the instability of the protein and an almost complete lack of its enzymatic activity. Subsequently, 27 further, independent Δacn clones were isolated, and 15 of them were found to contain distinct mutations in gltA, causing the loss of citrate synthase activity. A similar result was observed for mutants lacking the isocitrate dehydrogenase gene icd. In this case, 8 of 24 Δicd clones contained additional mutations in gltA. Indirect evidence was obtained that elevated intracellular citrate concentrations could be the cause of this selection pressure. Accordingly, the careful control of aconitase synthesis might have evolved due to the necessity to avoid inhibitory cytoplasmic citrate levels on the one hand and to prevent the excessive synthesis of an oxygen-sensitive protein requiring both iron and sulfur on the other hand.
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