ArticleJournal of bacteriology2011
Deletion of one nucleotide within the homonucleotide tract present in the hsdS gene alters the DNA sequence specificity of type I restriction-modification system NgoAV.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Restriction-modification systems are required forProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Restriction-modification systems are required forbioRxiv : the preprint server for biology · 2026Article
- Bacterial restriction-modification systems: mechanisms of defense against phage infection.Biophysics reports · 2025Article
- Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria.Frontiers in microbiology · 2023Review
- From genome structure to function: insights into structural variation in microbiology.Current opinion in microbiology · 2022Review
- Phase-variable Type I methyltransferase M.NgoAV fromFrontiers in microbiology · 2022Article
- Prevalence of phase variable epigenetic invertons among host-associated bacteria.Nucleic acids research · 2020Article
- Systematic Analysis of REBASE Identifies Numerous Type I Restriction-Modification Systems with Duplicated, DistinctmSystems · 2020Article
- DNA sequence repeats identify numerous Type I restriction-modification systems that are potential epigenetic regulators controlling phase-variable regulons; phasevarions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Genetic variation regulates the activation and specificity of Restriction-Modification systems in Neisseria gonorrhoeae.Scientific reports · 2019Article
- A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes.Nucleic acids research · 2018Article
- Phasevarions of Bacterial Pathogens: Methylomics Sheds New Light on Old Enemies.Trends in microbiology · 2018Review
- Article
- Phase variable DNA repeats inMicrobial genomics · 2016Article
- The Capricious Nature of Bacterial Pathogens: Phasevarions and Vaccine Development.Frontiers in immunology · 2016Review
- The complete methylome of Helicobacter pylori UM032.BMC genomics · 2015Article
- Article
- Removal of a frameshift between the hsdM and hsdS genes of the EcoKI Type IA DNA restriction and modification system produces a new type of system and links the different families of Type I systems.Nucleic acids research · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
As a result of a frameshift mutation, the hsdS locus of the NgoAV type IC restriction and modification (RM) system comprises two genes, hsdS(NgoAV1) and hsdS(NgoAV2). The specificity subunit, HsdS(NgoAV), the product of the hsdS(NgoAV1) gene, is a naturally truncated form of an archetypal specificity subunit (208 N-terminal amino acids instead of 410). The presence of a homonucleotide tract of seven guanines (poly[G]) at the 3' end of the hsdS(NgoAV1) gene makes the NgoAV system a strong candidate for phase variation, i.e., stochastic addition or reduction in the guanine number. We have constructed mutants with 6 guanines instead of 7 and demonstrated that the deletion of a single nucleotide within the 3' end of the hsdS(NgoAV1) gene restored the fusion between the hsdS(NgoAV1) and hsdS(NgoAV2) genes. We have demonstrated that such a contraction of the homonucleotide tract may occur in vivo: in a Neisseria gonorrhoeae population, a minor subpopulation of cells appeared to have only 6 guanines at the 3' end of the hsdS(NgoAV1) gene. Escherichia coli cells carrying the fused gene and expressing the NgoAVΔ RM system were able to restrict λ phage at a level comparable to that for the wild-type NgoAV system. NgoAV recognizes the quasipalindromic interrupted sequence 5'-GCA(N(8))TGC-3' and methylates both strands. NgoAVΔ recognizes DNA sequences 5'-GCA(N(7))GTCA-3' and 5'-GCA(N(7))CTCA-3', although the latter sequence is methylated only on the complementary strand within the 5'-CTCA-3' region of the second recognition target sequence.
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