ArticleNucleic acids research2011
Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Emerging Biohybrids of Aptamer-Based Nano-Biosensing Technologies for Effective Early Cancer Detection.Molecular diagnosis & therapy · 2024Review
- Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.ACS synthetic biology · 2021Article
- Optoribogenetic control of regulatory RNA molecules.Nature communications · 2020Article
- The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA-TetR regulator complex.Nucleic acids research · 2020Article
- Robust gene expression control in human cells with a novel universal TetR aptamer splicing module.Nucleic acids research · 2019Article
- A modular degron library for synthetic circuits in mammalian cells.Nature communications · 2019Article
- At the Intersection of Biomaterials and Gene Therapy: Progress in Non-viral Delivery of Nucleic Acids.Frontiers in bioengineering and biotechnology · 2019Review
- Aptamer-based targeted therapy.Advanced drug delivery reviews · 2018Review
- Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1.International journal of molecular sciences · 2018Article
- Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals.Nucleic acids research · 2017Article
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay.Nucleic acids research · 2016Article
- Selection and Biosensor Application of Aptamers for Small Molecules.Frontiers in chemistry · 2016Review
- Inhibition of Aggregation of Mutant Huntingtin by Nucleic Acid Aptamers In Vitro and in a Yeast Model of Huntington's Disease.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Aptamer-Drug Conjugates.Bioconjugate chemistry · 2015Review
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- A general design strategy for protein-responsive riboswitches in mammalian cells.Nature methods · 2014Article
- Aptamers: problems, solutions and prospects.Acta naturae · 2013Article
- Quantitative and simultaneous translational control of distinct mammalian mRNAs.Nucleic acids research · 2013Article
- Synthetic biology and personalized medicine.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aptamers binding proteins or small molecules have been shown to be versatile and powerful building blocks for the construction of artificial genetic switches. In this study, we present a novel aptamer-based construct regulating the Tet Off system in a tetracycline-independent manner thus achieving control of transgene expression. For this purpose, a TetR protein-inhibiting aptamer was engineered for use in mammalian cells, enabling the RNA-responsive control of the tetracycline-dependent transactivator (tTA). By rationally attaching the theophylline aptamer as a sensor, the inhibitory TetR aptamer and thus tTA activity became dependent on the ligand of the sensor aptamer. Addition of the small molecule theophylline resulted in enhanced binding to the corresponding protein in vitro and in inhibition of reporter gene expression in mammalian cell lines. By using aptamers as adaptors in order to control protein activity by a predetermined small molecule, we present a simple and straightforward approach for future applications in the field of Chemical Biology. Moreover, aptamer-based control of the widely used Tet system introduces a new layer of regulation thereby facilitating the construction of more complex gene networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.