ArticleNucleic acids research2012
Molecular architecture of a multifunctional MCM complex.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular Dissection of the Primase and Polymerase Activities of Deep-Sea Phage NrS-1 Primase-Polymerase.Frontiers in microbiology · 2021Article
- A cancer-associated point mutation disables the steric gate of human PrimPol.Scientific reports · 2019Article
- The Zn-finger domain of human PrimPol is required to stabilize the initiating nucleotide during DNA priming.Nucleic acids research · 2018Article
- Proteomic alterations in early stage cervical cancer.Oncotarget · 2018Article
- The Polyphyletic Origins of Primase-Helicase Bifunctional Proteins.Journal of molecular evolution · 2017Article
- Human PrimPol activity is enhanced by RPA.Scientific reports · 2017Article
- TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.Nature communications · 2016Article
- Molecular architecture of the recombinant human MCM2-7 helicase in complex with nucleotides and DNA.Cell cycle (Georgetown, Tex.) · 2016Article
- A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage.Nucleic acids research · 2016Article
- Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.Nucleic acids research · 2015Review
- Repriming of DNA synthesis at stalled replication forks by human PrimPol.Nature structural & molecular biology · 2013Article
- PrimPol, an archaic primase/polymerase operating in human cells.Molecular cell · 2013Article
- Article
- Visualization of the MCM DNA helicase at replication factories before the onset of DNA synthesis.Chromosoma · 2012Article
- Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.The Journal of biological chemistry · 2012Article
- PrimPol-A new polymerase on the block.Molecular & cellular oncologyReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. One of them is the replicative helicase, which is required for initiation and elongation phases. A MCM helicase found as a prophage in the genome of Bacillus cereus is fused with a primase domain constituting an integrative arrangement of two essential activities for replication. We have isolated this helicase-primase complex (BcMCM) showing that it can bind DNA and displays not only helicase and primase but also DNA polymerase activity. Using single-particle electron microscopy and 3D reconstruction, we obtained structures of BcMCM using ATPγS or ADP in the absence and presence of DNA. The complex depicts the typical hexameric ring shape. The dissection of the unwinding mechanism using site-directed mutagenesis in the Walker A, Walker B, arginine finger and the helicase channels, suggests that the BcMCM complex unwinds DNA following the extrusion model similarly to the E1 helicase from papillomavirus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.