Evidence map›Paper›PMID 21984262›Full record

ArticlePlanta2012

Functional characterization of an acidic SK(3) dehydrin isolated from an Opuntia streptacantha cDNA library.

A E Ochoa-Alfaro, M Rodríguez-Kessler, M B Pérez-Morales, P Delgado-Sánchez, C L Cuevas-Velazquez, G Gómez-Anduro, J F Jiménez-Bremont

Abstract read
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In one paragraph

Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
7.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 53 citations in OpenAlex.

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  6. Heterologous Expression of ThreePlants (Basel, Switzerland) · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

A E Ochoa-AlfaroDivision de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica, Camino a la Presa de San Jose 2055, Apartado Postal 3-74 Tangamanga, CP 78216 San Luis Potosi, SLP, Mexico.
M Rodríguez-Kessler
M B Pérez-Morales
P Delgado-Sánchez
C L Cuevas-Velazquez
G Gómez-Anduro
J F Jiménez-Bremont
Institute for Scientific and Technological Research · MXAutonomous University of San Luis Potosí · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cactus pears are succulent plants of the Cactaceae family adapted to extremely arid, hot and cold environments, making them excellent models for the study of molecular mechanisms underlying abiotic stress tolerance. Herein, we report a directional cDNA library from 12-month-old cladodes of Opuntia streptacantha plants subjected to abiotic stresses. A total of 442 clones were sequenced, representing 329 cactus pear unigenes, classified into eleven functional categories. The most abundant EST (unigen 33) was characterized under abiotic stress. This cDNA of 905 bp encodes a SK(3)-type acidic dehydrin of 248 amino acids. The OpsDHN1 gene contains an intron inserted within the sequence encoding the S-motif. qRT-PCR analysis shows that the OpsDHN1 transcript is specifically accumulated in response to cold stress, and induced by abscisic acid. Over-expression of the OpsDHN1 gene in Arabidopsis thaliana leads to enhanced tolerance to freezing treatment, suggesting that OpsDHN1 participates in freezing stress responsiveness. Generation of the first EST collection for the characterization of cactus pear genes constitutes a useful platform for the understanding of molecular mechanisms of stress tolerance in Opuntia and other CAM plants.

Indexed as

Gene LibraryCold TemperatureGene Expression Regulation, PlantOpuntiaPlant ProteinsPlant Proteins

Identifiers

PMID21984262
OpenAlexW2079069135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.