ArticleNature methods2011
Artificial niche microarrays for probing single stem cell fate in high throughput.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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Who cites it
144 citing papers in PubMed.
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- Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers.Materials today. Bio · 2024Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- The mechanics of the retina: Müller glia role on retinal extracellular matrix and modelling.Frontiers in medicine · 2024Review
- Rapid prototyping of high-resolution large format microfluidic device through maskless image guided in-situ photopolymerization.Nature communications · 2023Article
- Identification of the Differentiation Stages of Living Cells from the Six Most Immature Murine Hematopoietic Cell Populations by Multivariate Analysis of Single-Cell Raman Spectra.Analytical chemistry · 2022Article
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- Engineering multiscale structural orders for high-fidelity embryoids and organoids.Cell stem cell · 2022Review
- A deep-learning-based workflow to deal with the defocusing problem in high-throughput experiments.Bioactive materials · 2022Article
- Decellularized extracellular matrix mediates tissue construction and regeneration.Frontiers of medicine · 2022Review
- High-Content Screening and Analysis of Stem Cell-Derived Neural Interfaces Using a Combinatorial Nanotechnology and Machine Learning Approach.Research (Washington, D.C.) · 2022Article
- Hydrogels Containing Gradients in Vascular Density Reveal Dose-Dependent Role of Angiocrine Cues on Stem Cell Behavior.Advanced functional materials · 2021Article
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.The Journal of cell biology · 2021Article
- Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays.Fluids and barriers of the CNS · 2021Article
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021Review
- On-demand retrieval of cells three-dimensionally seeded in injectable thioester-based hydrogels.RSC advances · 2021Article
84 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the regulatory role of niches in maintaining stem-cell fate, multifactorial in vitro models are required. These systems should enable analysis of biochemical and biophysical niche effectors in a combinatorial fashion and in the context of a physiologically relevant cell-culture substrate. We report a microengineered platform comprised of soft hydrogel microwell arrays with modular stiffness (shear moduli of 1-50 kPa) in which individual microwells can be functionalized with combinations of proteins spotted by robotic technology. To validate the platform, we tested the effect of cell-cell interactions on adipogenic differentiation of adherent human mesenchymal stem cells (MSCs) and the effect of substrate stiffness on osteogenic MSC differentiation. We also identified artificial niches supporting extensive self-renewal of nonadherent mouse neural stem cells (NSCs). Using this method, it is possible to probe the effect of key microenvironmental perturbations on the fate of any stem cell type in single cells and in high throughput.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.