ArticleBlood cells, molecules & diseases2012
Angiopoietin-like-2 and -3 act through their coiled-coil domains to enhance survival and replating capacity of human cord blood hematopoietic progenitors.
Article in Blood cells, molecules & diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Angptl2 is a Marker of Cellular Senescence: The Physiological and Pathophysiological Impact of Angptl2-Related Senescence.International journal of molecular sciences · 2021Review
- ANGPTL2 Induces Synovial Inflammation via LILRB2.Inflammation · 2021Article
- Effect of ANGPTL7 on Proliferation and Differentiation of MC3T3-E1 Cells.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Angiopoietin-Like Proteins in Angiogenesis, Inflammation and Cancer.International journal of molecular sciences · 2018Review
- Shared liver-like transcriptional characteristics in liver metastases and corresponding primary colorectal tumors.Journal of Cancer · 2018Article
- High Circulating Levels of ANGPTL2: Beyond a Clinical Marker of Systemic Inflammation.Oxidative medicine and cellular longevity · 2017Review
- Article
- ANGPTL3 is a novel biomarker as it activates ERK/MAPK pathway in oral cancer.Cancer medicine · 2015Article
- Angiopoietin-like protein 2 regulates endothelial colony forming cell vasculogenesis.Angiogenesis · 2014Article
- Low-dose insulin-like growth factor binding proteins 1 and 2 and angiopoietin-like protein 3 coordinately stimulate ex vivo expansion of human umbilical cord blood hematopoietic stem cells as assayed in NOD/SCID gamma null mice.Stem cell research & therapy · 2014Article
- Cloning, expression and purification of functionally active human angiopoietin-like protein 2.SpringerPlus · 2014Article
- Angiopoietin-like protein 3 promotes preservation of stemness during ex vivo expansion of murine hematopoietic stem cells.PloS one · 2014Article
- Angiopoietin-like 2 promotes atherogenesis in mice.Journal of the American Heart Association · 2013Article
- Enhancing engraftment of cord blood cells via insight into the biology of stem/progenitor cell function.Annals of the New York Academy of Sciences · 2012Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Several angiopoietin-like (ANGPTL) molecules have been implicated in enhancement of ex-vivo expansion of murine and human (hu) hematopoietic stem cells, but there are no reports on hematopoietic progenitor cells (HPCs). We assessed purified recombinant endotoxin-free hu ANGPTL-2 Coiled-Coil (CC), -3, -3CC, -3 fibrinogen-like domain (FLD), -4, -4CC, -5CC, -6 and -7 for effects on proliferation and survival of HPCs from hu cord blood (CB). None of the ANGPTL molecules stimulated CB HPC proliferation, or enhanced or inhibited colony formation of CB HPC stimulated by various growth factors. However, ANGPTL-2CC, -3, and -3CC significantly enhanced survival of HPC (CFU-GM, BFU-E, CFU-GEMM) subjected to delayed addition of growth factors. Survival enhancing effects of ANGPTL-3 were neutralized by purified anti-ANGPTL-3, but not by anti-ANGPTL-4, -6, or -7. ANGPTL-2CC, -3, and -3CC, but not -4, -6, or -7 also enhanced replating capacity of single CB CFU-GEMM colonies, an estimate of the self-renewal capabilities of HPCs, by greater than 2 fold. Effects of at least ANGPTL-3CC may in part be mediated through phosphorylation of ERK. The ANGPTL molecules did not influence ex-vivo expansion of hu CB CD34(+) cells, alone, or in combination with SCF, TPO, Flt3-ligand, with or without IL-3. Thus, among ANGPTL family members, ANGPTL-2 and -3 had enhancing activities on human HPC survival and replating activity, effects requiring the CC domain of the ANGPTL molecules. This information is of relevance to hu HPC regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.