ArticleBiomaterials2012
Whispering gallery mode biosensor quantification of fibronectin adsorption kinetics onto alkylsilane monolayers and interpretation of resultant cellular response.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Microfluidics and Nanofluidics in Strong Light-Matter Coupling Systems.Nanomaterials (Basel, Switzerland) · 2024Review
- Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology.ACS photonics · 2024Review
- Sensitivity Equalization and Dynamic Range Expansion with Multiple Optofluidic Microbubble Resonator Sensors.Biosensors · 2023Article
- Mimicking the Tendon Microenvironment to Enhance Skeletal Muscle Adhesion and Longevity in a Functional Microcantilever Platform.ACS biomaterials science & engineering · 2023Article
- Temporal Characterization of Neuronal Migration Behavior on Chemically Patterned Neuronal Circuits in a DefinedACS biomaterials science & engineering · 2018Article
- Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges.Advanced healthcare materials · 2018Review
- Self-contained, low-cost Body-on-a-Chip systems for drug development.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Biosensing by WGM Microspherical Resonators.Sensors (Basel, Switzerland) · 2016Review
- Applications of Optical Microcavity Resonators in Analytical Chemistry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
- PEG Functionalization of Whispering Gallery Mode Optical Microresonator Biosensors to Minimize Non-Specific Adsorption during Targeted, Label-Free Sensing.Sensors (Basel, Switzerland) · 2015Article
- Whispering gallery mode sensors.Advances in optics and photonics · 2015Article
- Ratiometric detection of oligonucleotide stoichiometry on multifunctional gold nanoparticles by whispering gallery mode biosensing.The Analyst · 2015Article
- Stand-off biodetection with free-space coupled asymmetric microsphere cavities.Sensors (Basel, Switzerland) · 2015Article
- Label-free, single molecule resonant cavity detection: a double-blind experimental study.Sensors (Basel, Switzerland) · 2015Article
- Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation.Biomaterials · 2015Article
- Single-molecule nucleic acid interactions monitored on a label-free microcavity biosensor platform.Nature nanotechnology · 2014Article
- Myelination and node of Ranvier formation on sensory neurons in a defined in vitro system.In vitro cellular & developmental biology. Animal · 2013Article
- Two cell circuits of oriented adult hippocampal neurons on self-assembled monolayers for use in the study of neuronal communication in a defined system.ACS chemical neuroscience · 2013Article
- Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices.Nanophotonics (Berlin, Germany) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A Whispering Gallery Mode (WGM) biosensor was constructed to measure the adsorption of protein onto alkysilane self-assembled monolayers (SAMs) at solution concentrations unattainable with other techniques. The high sensitivity was provided by a WGM resonance excited in a silica microsphere that was functionalized with alkylsilane SAMs and integrated in a microfluidic flow cell under laminar flow conditions. It was found that FN adsorbed at biologically relevant surface densities, however, the adsorption kinetics and concentration dependent saturation values varied significantly from work published utilizing alkanethiol SAMs. Mathematical models were applied to the experimental results to interpret the observed kinetics of FN adsorption. Embryonic hippocampal neurons and skeletal myoblasts were cultured on the modified surfaces, a live--dead assay was used to determine the viability of the FN surfaces for cell culture, and major differences were noted in the biological response to the different SAMs. The high sensitivity and simplicity of the WGM biosensor, combined with its ability to quantify the adsorption of any dilute protein in a label-free assay, establishes the importance of this technology for the study of surface accretion and its effect on cellular function, which can affect biomaterials for both in vivo and in vitro applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.